TRPM4通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响

doi:10.13701/j.cnki.kqyxyj.2020.05.021

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (5): 490-495.DOI: 10.13701/j.cnki.kqyxyj.2020.05.021

• 口腔生物学研究 • 上一篇

TRPM4通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响

王薛藤^1,2, 聂敏海^1,2, 左东川³, 邓浩², 曾锦^2,4*

1.西南医科大学附属口腔医院牙周黏膜科四川泸州 646000;
2.西南医科大学口颌面修复重建和再生实验室四川泸州 646000;
3.西南医科大学心血管医学研究所四川泸州 646000;
4.西南医科大学附属口腔医院正畸科四川泸州 646000

收稿日期:2019-09-09 出版日期:2020-06-16 发布日期:2020-06-18
通讯作者: ^* 曾锦,E-mail: zengjin@swmu.edu.cn
作者简介:王薛藤(1992~ ),女,四川乐山人,硕士在读,医师,研究方向:口腔黏膜病的预防与治疗。
基金资助:
四川省科技厅课题(川科技[2018]26号,项目编号2018JY0401);西南医科大学市-校-附属口腔医院联合项目(编号:0800103009);西南医科大学应用基础研究计划项目(编号:2017-ZRZD-001)

Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts

WANG Xueteng^1,2, NIE Minhai^1,2, ZUO Dongchuan³, DENG Hao², ZENG Jin^2,4*

1. Department of Periodontal Mucosa, Affiliated Stomatological Hospital, Southwestern Medical University, Luzhou 646000, China;
2. Oralfacial Reconstruction and Regeneration Laboratory, Luzhou 646000, China;
3. Cardiovascular Research Institute, Southwest Medical University, Luzhou 646000, China;
4. Department of Orthodontics, Affiliated Stomatological Hospital, Southwestern Medical University, Luzhou 646000, China

Received:2019-09-09 Online:2020-06-16 Published:2020-06-18

摘要/Abstract

摘要： 目的:验证TRPM4(transient receptor potential melastatin-4)在口腔颊黏膜成纤维细胞的表达,探讨其对人口腔颊黏膜成纤维细胞迁移与增殖中的作用。方法:收集人正常颊黏膜组织进行原代细胞提取与原代培养;采用RT-PCR和细胞免疫荧光方法检测成纤维细胞中TRPM4的表达。全细胞膜片钳记录成纤维细胞TRPM4通道的全细胞电流。MTT法和细胞划痕实验分别检测应用TRPM4通道特异性阻断剂(9-菲酚)或特异性siRNA抑制TRPM4通道的功能对成纤维细胞增殖和迁移的影响。结果:人口腔颊黏膜成纤维细胞功能性表达TRPM4。抑制TRPM4通道的功能能够明显降低成纤维细胞的增殖和迁移能力。结论:TRPM4通道参与成纤维细胞增殖以及迁移能力的调控。

关键词: 颊黏膜成纤维细胞, TRPM4, 细胞增殖, 细胞迁移

Abstract: Objective: To verify the expression of TRPM4 in buccal mucosal fibroblasts and explore its effects on migration and proliferation of buccal fibroblasts. Methods: Human buccal mucosa fibroblasts were isolated and cultured in vitro, and the expression of TRPM4 in fibroblasts was evaluated by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence. Whole cell patch clamp were performed to record the whole cell currents of TRPM4 channels. The effects of 9-phenanthrene or TRPM4 specific siRNA on the proliferation and migration of fibroblasts were estimated by MTT assay and cell scratch test, respectively. Results: TRPM4 was functional expressed in human buccal mucosa fibroblasts. Suppression the function of TRPM7 channel by 9-phenanthrene or specific siRNA inhibited the proliferation and migration capability of the cells. Conclusion: TRPM4 channels is involved in the regulation of proliferation and migration in human buccal mucosa fibroblasts.

Key words: buccal mucosal fibroblast, TRPM4, cell proliferation, cell migration

王薛藤, 聂敏海, 左东川, 邓浩, 曾锦. TRPM4通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J]. 口腔医学研究, 2020, 36(5): 490-495.

WANG Xueteng, NIE Minhai, ZUO Dongchuan, DENG Hao, ZENG Jin. Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts[J]. Journal of Oral Science Research, 2020, 36(5): 490-495.

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TRPM4通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响

Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts

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