高脂饮食对小鼠颞下颌关节骨关节炎的影响

doi:10.13701/j.cnki.kqyxyj.2023.08.009

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (8): 707-714.DOI: 10.13701/j.cnki.kqyxyj.2023.08.009

高脂饮食对小鼠颞下颌关节骨关节炎的影响

赵琛^1,2,3*, 商庆龙¹, 赵甜甜¹, 张毕承⁴, 苏彤⁴, 梁蓓蓓^1,2,3, 刘博宇^1,2,3

1.河北医科大学口腔医学院·口腔医院修复科河北石家庄 050017;
2.河北省口腔医学重点实验室河北石家庄 050017;
3.河北省口腔疾病临床医学研究中心河北石家庄 050017;
4.河北医科大学临床学院河北石家庄 050031

收稿日期:2023-05-15 发布日期:2023-08-17
通讯作者: *赵琛,E-mail:zhaochen36@163.com
作者简介:赵琛(1977~ ),女,四川宣汉人,博士,副主任医师,研究方向:咬合病与颞下颌关节紊乱病。
基金资助:
河北省医学科学研究课题计划指导性课题(编号:20201193)2019年河北省财政厅老年病防治科研项目(编号:361029)2022年河北省政府资助临床医学优秀人才培养和基础课题研究项目(编号:361029)河北省大学生创新创业训练计划项目(编号:S202113415011)

Effect of High-fat Diet on Osteoarthritis of Temporomandibular Joint in Mice

ZHAO Chen^1,2,3*, SHANG Qinglong¹, ZHAO Tiantian¹, ZHANG Bicheng⁴, SU Tong⁴, LIANG Beibei^1,2,3, LIU Boyu^1,2,3

1. Department of Prosthodontics, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China;
2. Hebei Key Laboratory of Stomatology, Shijiazhuang 050017, China;
3. Hebei Clinical Research Center For Oral Disease, Shijiazhuang 050017, China;
4. College of Clinical, Hebei Medical University, Shijiazhuang 050031, China

Received:2023-05-15 Published:2023-08-17

摘要/Abstract

摘要： 目的: 通过检测髁突软骨内IL-1β、TNF-α、VEGF的表达,探讨高脂饮食对小鼠颞下颌关节骨关节炎的影响。方法: 48只小鼠随机分为正常饮食(ND)组、常规建模(MF+ND)组、高脂建模(MF+HFD)组、混合建模(MF+MD)组。采用前牙单侧反牙合法建立异常咬合模型,建模后的第2、4、6、8 周处死小鼠取双侧颞下颌关节。通过HE和番红-O固绿染色进行病理观察及评分。免疫组织化学染色检测髁突软骨内IL-1β、TNF-α、VEGF的表达。结果: HE和番红-O固绿染色结果显示,建模组可见髁突软骨破坏,Mankin评分增高;而MF+HFD组破坏更加严重,Mankin评分更高;免疫组织化学染色结果显示,各建模组IL-1β、TNF-α和VEGF的阳性细胞数明显增加,并且MF+ND、MF+HFD和MF+MD三组IL-1β、TNF-α、VEGF阳性细胞表达比较均具有显著差异(P＜0.05)。结论: 长期的高脂饮食可以加重咬合异常所诱导的颞下颌关节骨关节炎小鼠的髁突软骨破坏,加重小鼠颞下颌关节骨关节炎的程度。

关键词: 高脂饮食, 颞下颌关节骨关节炎, 血管内皮生长因子, 肿瘤坏死因子-α, 白细胞介素-1β

Abstract: Objective: To explore the effect of high-fat diet on temporomandibular joints osteoarthritis of the mouse by examining the expression of IL-1β, TNF-α, and VEGF. Methods: Forty-eight mice were randomly divided into regular diet(ND)group, abnormal occlusion with regular diet(MF+ND)group,abnormal occlusion with high fat(MF+HFD)group, and abnormal occlusion with mixed diet(MF+MD)group. The study used the unilateral anterior crossbite method to establish the mechanical force model. At the end of the 2nd, 4th, 6th, and 8th week after the model was established, the bilateral temporomandibular joints were collected. HE staining and saffron O staining of condylar cartilage were observed and scored pathologically. The expression of IL-1β, TNF-α, and VEGF of the condylar cartilage was detected by immunohistochemical staining. Results: In the MF+ND group, condylar cartilage destruction was visible and Mankin score increased, while MF+HFD group had more severe destruction and higher Mankin score. Immunohistochemical staining showed that the number of positive cells identified for IL-1β, TNF-α, and VEGF was significantly higher in each model group. Moreover, the expressions of IL-1β, TNF-α, and VEGF positive cells in the MF+ND, MF+HFD, and MF+MD groups were significantly different (P<0.05). Conclusion: High-fat diet can aggravate the condylar cartilage destruction in temporomandibular joints osteoarthritis in mice induced by occlusal abnormalities and aggravate the degree of temporomandibular joints osteoarthritis in the mice.

Key words: high fat diet, temporomandibular joint osteoarthritis, vascular endothelial growth factor, tumor necrosis factor-α, interleukin-1β

赵琛, 商庆龙, 赵甜甜, 张毕承, 苏彤, 梁蓓蓓, 刘博宇. 高脂饮食对小鼠颞下颌关节骨关节炎的影响[J]. 口腔医学研究, 2023, 39(8): 707-714.

ZHAO Chen, SHANG Qinglong, ZHAO Tiantian, ZHANG Bicheng, SU Tong, LIANG Beibei, LIU Boyu. Effect of High-fat Diet on Osteoarthritis of Temporomandibular Joint in Mice[J]. Journal of Oral Science Research, 2023, 39(8): 707-714.

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高脂饮食对小鼠颞下颌关节骨关节炎的影响

Effect of High-fat Diet on Osteoarthritis of Temporomandibular Joint in Mice

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