负载D-缬氨酸水凝胶对大鼠实验性牙周炎的影响

doi:10.13701/j.cnki.kqyxyj.2020.07.012

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (7): 658-663.DOI: 10.13701/j.cnki.kqyxyj.2020.07.012

负载D-缬氨酸水凝胶对大鼠实验性牙周炎的影响

王昆于¹, 孙毓言¹, 张慧彦^1,2, 齐晓爽^1,2, 张震阳^1,2, 乔树伟^1,2, 向镜成¹, 丁佳鑫¹, 孟维艳^1,2*

1.吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130000;
2.吉林大学口腔医院口腔种植中心吉林长春 130000

收稿日期:2019-10-24 出版日期:2020-07-28 发布日期:2020-07-24
通讯作者: 孟维艳,E-mail:mengsitong66@163.com
作者简介:王昆于(1998~),男,重庆人,本科在读,研究方向:牙周炎及其治疗。
基金资助:
大学生创新训练项目(编号:2018B3312);国家自然科学基金(编号:81571007);吉林省科技发展自然科学基金(编号:20180101121JC)

Effects of D-valine Loaded Hydrogel on Rat Periodontitis

WANG Kunyu¹, SUN Yuyan¹, ZHANG Huiyan^1,2, QI Xiaoshuang^1,2, ZHANG Zhenyang^1,2, QIAO Shuwei^1,2, XIANG Jingcheng¹, DING Jiaxin¹, MENG Weiyan^1,2*

1. Jilin Province Key Laboratory of Tooth Development and Jaw Remodeling and Regeneration, Changchun 130000, China;
2. Dental Implant Center, Stomatological Hospital, Jilin University, Changchun 130000, China

Received:2019-10-24 Online:2020-07-28 Published:2020-07-24

摘要/Abstract

摘要： 目的: 探讨负载D-缬氨酸水凝胶对大鼠实验性牙周炎及相关炎症因子表达的影响。方法: 将30只SD大鼠分为阴性对照组(A组)和牙周炎模型组(B组),再在模型组基础上给予不同药物,分为派丽奥组(C组,阳性对照组)、负载D-缬氨酸水凝胶组(D组)、空白水凝胶组(E组),共分为5组,每组6只。成功构建牙周炎模型后,测量各组给药后牙龈指数(GI)、菌斑指数(PI)、牙周袋深度(PD),CBCT测量牙槽骨吸收值(ABL),HE染色观察牙周组织病理变化,ELISA检测IL-1β、IL-6、IL-10、TNF-α水平。结果: 模型组牙周指数,IL-1β、IL-6、TNF-α均升高,IL-10降低;治疗后C、D组GI、PI、ABL明显低于模型组(P<0.05),3组PD值与模型组比较差异无统计学意义(P>0.05);D组IL-1β、IL-6、TNF-a水平明显低于模型组(P<0.01),进一步治疗D组IL-6、TNF-α与A组比较差异无统计学意义(P>0.05),D组IL-10明显高于模型组(P<0.01)。结论: 负载D-缬氨酸水凝胶可降低炎症因子水平,减少牙槽骨吸收,对改善牙周炎具有较好疗效,可能与抑制牙周致病菌有关。

关键词: 牙周炎, 水凝胶, D-缬氨酸

Abstract: Objective: To investigate the effect of D-valine hydrogel on experimental periodontitis and related inflammatory factors in rats. Methods: Thirty SD rats were divided into five groups: negative control group (group A), periodontitis model group (group B), the periodontitis with PaiLi (group C, positive control group), the periodontitis with D-valine hydrogel (group D), and the periodontitis with blank hydrogel (group E). The gingival index (GI), plaque index (PI), periodontal pocket depth (PD), and alveolar bone absorption (ABL) were measured by CBCT. Pathological changes of periodontal tissues were observed by HE staining, and the levels of IL-1β, IL-6, IL-10, and TNF-α were detected by ELISA. Results: The periodontal index, IL-1β, IL-6, and TNF-a in the model group were all increased and IL-10 was decreased. The GI, PI, and ABL in the group C and D were significantly lower than those in the group B (P<0.05), and the PD values in the groups C, D, and E were not significantly different from those in the group B (P>0.05). The IL-1 β, IL-6, and TNF-a levels in the group D were significantly lower than those in the group B (P<0.01), while the IL-6, TNF-a levels in the group D were not significantly different from those in the group A (P>0.05). And the level of IL-10 in group D was significantly higher than that of group B (P<0.01). Conclusion: D-valine hydrogel can reduce inflammatory factor level, reduce alveolar bone resorption, and improve periodontitis, which may be related to periodontal pathogens.

Key words: periodontitis, hydrogel, D-valine

王昆于, 孙毓言, 张慧彦, 齐晓爽, 张震阳, 乔树伟, 向镜成, 丁佳鑫, 孟维艳. 负载D-缬氨酸水凝胶对大鼠实验性牙周炎的影响[J]. 口腔医学研究, 2020, 36(7): 658-663.

WANG Kunyu, SUN Yuyan, ZHANG Huiyan, QI Xiaoshuang, ZHANG Zhenyang, QIAO Shuwei, XIANG Jingcheng, DING Jiaxin, MENG Weiyan. Effects of D-valine Loaded Hydrogel on Rat Periodontitis[J]. Journal of Oral Science Research, 2020, 36(7): 658-663.

参考文献

[1] 吴芸菲,张莉,张旭凤,等.烟草提取物对牙周炎小鼠牙周组织TLR4炎症通路的影响[J].口腔医学研究,2019,35(7):651-656.
[2] Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms [J]. Clin Microbiol Rev, 2002, 15(2):167-193.
[3] Araujo AA, Souza TO, Moura LM, et al. Effect of telmisartan on levels of IL-1, TNF-α, down-regulated COX-2, MMP-2, MMP-9 and RANKL/RANK in an experimental periodontitis model [J]. J Clin Periodontol, 2013, 40(12):1104-1111.
[4] Petersen PE, Ogawa H. The global burden of periodontal disease:towards integration with chronic disease prevention and control [J]. Periodontol 2000,2012, 60(1):15-39.
[5] Terlemez S, Eryilmaz U, Tokgoz Y, et al. Kounis syndrome caused by metronidazole-a case of 14 year-old boy [J]. Int J Cardiol, 2015, 179:222-224.
[6] 唐彩金,曾启新,方梅飞,等.消退素D1治疗实验性大鼠牙周炎的研究[J].口腔医学研究,2017,33(12):1270-1273.
[7] Kulik EM. Development of resistance of mutans stretococci and porphyromonas gingivalis to chlorhexidine digluconate and amine fluoride/stannous fluoride-containing mothrinses,in vitro [J]. Clin Oral Investig, 2015, 19(6):1547-1553.
[8] Pimentel SP. Protective effect of topical Cordia Verbenacea in a rat periodontitis model:immune-inflammatory,antibacterial and morphometric assays [J]. BMC Complement Altern Med, 2012, 12:224.
[9] Lam H, Oh DC, Cava F, et al. D-amino acids govern statinary phase cell wall remodeling in bacteria [J]. Science, 2009, 325(5947):1552-1555.
[10] Kolodkin-Gal I, Romero D, Cao S, et al. D-amino acids trigger biofilm disassembly [J]. Science, 2010, 328(5978):627-629.
[11] 郭家平,李欧,符旭东.盐酸多西环素微球温敏凝胶剂的表征及对大鼠牙周炎的药效评价[J].临床口腔医学杂志,2016,32(12):714-717.
[12] 邓咏梅,王辉,何松霖,等.盐酸米诺环素纳米脂质体治疗大鼠实验性牙周炎的疗效观察[J].实用口腔医学杂志,2018,34(6):753-757.
[13] 顾中一,徐晓薇,王卓然,等.载 EPO壳聚糖水凝胶促牙周组织再生的研究[J].口腔生物医学,2017,8(3):127-131.
[14] 孙俊毅,朱春晖,刘瑾,等.盐酸小檗碱调节大鼠实验性牙周炎牙龈组织MMPs/TIMP表达变化及其机制[J].西安交通大学学报(医学版),2016,37(4):551-555.
[15] 路瑞芳,冯琳,高学军,等.侵袭性牙周炎龈沟液中有机酸与牙龈卟啉单胞菌和齿垢密螺旋体的关系[J].北京大学学报( 医学版),2013,45(1):12-16.
[16] Sumita J, Richard P. Contribution of Porphyromonas gingivalis lipopolysachharide to periodontitis[J].Periodontol 2000, 2010, 54(1):53-70.
[17] 瞿凤环,邓婧,潘克清,等.局部注射LPS对慢性牙周炎模型大鼠牙髓、修复性牙本质形成的影响[J].山东医药,2012,52(18):36-37.
[18] Shinwari MS, Tanwir F, Hyder PR, et al. Host modulation therapeutics in periodontics: role as an adjunctive periodontal therapy [J]. J Coll Physicians Surg Pak,2014, 24(9):676-684.
[19] 张晓敏,郭恪,任春霞,等.牙周炎骨丧失机制中关于炎症和解偶联的研究[J].国际口腔医学杂志,2013,40(2):206-208.
[20] Hajishengallis G. Immunomicrobial pathogenesis of periodontitis:keystones,pathobionts,and host response [J].Trends Immunol,2014,35(1):3-11.
[21] Garlet GP, Martins W Jr, Fonseca BA, et al. Matrix metalloproteinases, their physiological inhibitors and osteoclast factors are differentially regulated by the cytokine profile in human periodontal disease [J]. J Clin Periodontol, 2004, 31(8):671-679.
[22] Lee DE,Kim JH,Choi SH,et al. Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts [J]. J Periodontal Res,2015,50(2):256-264.
[23] Silva N,Abusleme L,Bravo D, et al. Host response mechanisms in periodontal diseases [J]. J Appl Oral Sci,2015,23(3) :329-355.
[24] Rybtke M, Hultqvist LD, Givskov M, et al. Pseudomonas aeruginosa biofilm infections: community structure, antimicrobial tolerance and immune response [J]. J Mli Biol, 2015, 427(23):36828-26845.
[25] Herzberg M, Kang S, Elimelech M. Role of extracellular polymeric substances (EPS) in biofouling of reverse osmosis membranes [J]. Environ Sci Technol, 2009, 43(12):4393-4398.
[26] Pöllänen MT, Paino A, Ihalin R. Environmental stimuli shape biofilm formation and the virulence of periodontal pathogens [J]. Int J Mol Sci, 2013, 14(8):17221-17237.
[27] Kolodkin-Gal I, Romero D, Cao S, et al. Damino acids trigger biofilm disassembly [J]. Science, 2010, 328(5978):627-629.
[28] Leiman SA, May JM, Lebar MD, et al. Amino acids indirectly inhibit biofilm formation in Bacillus subtilis by interfering with protein synthesis[J].J Bacteriol, 2013, 195(23):5391-5395.
[29] Sanchez Z, Tani A, Kimbara K. Extensive reduction of cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with a D-amino acid mixture[J]. Appl Environ Microbiol, 2013, 79(4):1396-1399.
[30] Hochbaum AI, Kolodkin-Gal I, Foulston L, et al. Inhibitory effects of D-amino acids on Staphylococcus aureus biofilm development [J]. J Bacteriol, 2011, 193(20):5616-5622.
[31] Qi H, Li B, Wang H, et al. Effects of dvaline on periodontal or periimplant pathogens: Porphyromonas gingivalis biofilm [J]. J Periodontol, 2018, 89(3):303-314.
[32] Freire MO, Van Dyke TE. Natural resolution of inflammation [J]. Periodontol 2000, 2013, 63(1):149-164.

负载D-缬氨酸水凝胶对大鼠实验性牙周炎的影响

Effects of D-valine Loaded Hydrogel on Rat Periodontitis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	周岳, 申玉芹. 透明质酸凝胶联合Nd:YAG激光对老年牙周炎患者牙根敏感的实验研究及临床疗效观察[J]. 口腔医学研究, 2021, 37(1): 39-42.
[2]	鲁皓, 李金凤, 苏旭, 戴江红, 陈晓涛. 负性情绪与慢性牙周炎的相关性研究[J]. 口腔医学研究, 2021, 37(1): 43-47.
[3]	刘悦, 王雷. 他汀类药物与牙周炎关系的研究进展[J]. 口腔医学研究, 2020, 36(9): 811-813.
[4]	张黎, 刘育菘, 吴芸菲, 符起亚. 基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响[J]. 口腔医学研究, 2020, 36(9): 844-849.
[5]	王岩松, 刘建林, 陈晓霞 ,白姝婷. Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究[J]. 口腔医学研究, 2020, 36(8): 731-735.
[6]	齐晓爽, 孟维艳. 用于牙周炎治疗的自噬调节物研究现状[J]. 口腔医学研究, 2020, 36(7): 616-618.
[7]	张慧彦, 李保胜, 张震阳, 韩春雨, 李尊泰, 齐晓爽, 付齐月, 孟维艳. D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用[J]. 口腔医学研究, 2020, 36(7): 648-653.
[8]	刘君瑜, 肖宇, 王翔宇, 刘艳, 李玉芳, 柳海凤, 陈宇, 包崇云. 血糖敏感型水凝胶治疗糖尿病大鼠牙周炎的实验研究[J]. 口腔医学研究, 2020, 36(6): 567-571.
[9]	吴芸菲, 张黎, 张旭凤, 符起亚. 烟草提取物辅酶Q10对牙周炎小鼠Treg/T17细胞免疫平衡的影响[J]. 口腔医学研究, 2020, 36(6): 572-576.
[10]	周伟, 汤雅, 李厚轩. 南京地区侵袭性牙周炎近6年就诊情况调查分析[J]. 口腔医学研究, 2020, 36(5): 473-476.
[11]	臧文丽, 刘俊超, 潘亚萍. 丁酸对牙周炎的影响机制研究进展[J]. 口腔医学研究, 2020, 36(4): 318-320.
[12]	田源, 陈靖, 曾凤娇, 于航, 刘霞, 刘建国, 白国辉. SD大鼠经鼻黏膜免疫牙周炎基因疫苗pVAX1-HA2-FimA-(IL-15)后Tfh细胞的变化[J]. 口腔医学研究, 2020, 36(4): 350-354.
[13]	丁小函, 岳轶云, 夏博园, 李艳, 刘歆婵, 武洲, 于维先. Mongersen对大鼠牙周炎抗炎效果观察[J]. 口腔医学研究, 2020, 36(4): 382-386.
[14]	杨磊, 郭留云, 程志芬, 李碧浩. Shh蛋白与慢性牙周炎炎症程度的相关性研究[J]. 口腔医学研究, 2020, 36(2): 131-134.
[15]	韩亚琨, 于程程, 于艳. 牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点[J]. 口腔医学研究, 2020, 36(2): 135-138.