基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响

doi:10.13701/j.cnki.kqyxyj.2020.09.011

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (9): 844-849.DOI: 10.13701/j.cnki.kqyxyj.2020.09.011

基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响

张黎^1,2*, 刘育菘¹, 吴芸菲³, 符起亚¹

1.海南医学院口腔医学院海南海口 571101;
2.海南光华口腔医院口腔正畸科海南海口 570203;
3.海南医学院第一附属医院口腔科海南海口 571101

收稿日期:2020-01-13 出版日期:2020-09-28 发布日期:2020-09-15
通讯作者: *张黎,E-mail: zhanglili0437@163.com
作者简介:张黎(1980~),女,海南海口人,博士,副主任医师,主要从事正畸与牙周的研究。
基金资助:
海南省高等学校科学研究重点项目(编号:Hnky2019ZD-31)

Effects of Puerarin on Alveolar Bone Resorption and OPG/RANKL/RANK Pathway in Rats with Periodontitis Based on IL-23/Th17 Inflammatory Axis

ZHANG Li^1,2*, LIU Yusong¹, WU Yunfei³, FU Qiya¹

1. School of Stomatology, Hainan Medical College, Haikou 571101, China;
2. Department of Orthodontics, Hainan Guanghua Stomatological Hospital, Haikou 570203, China;
3. Department of Stomatology, the First Affiliated Hospital of Hainan Medical College, Haikou 571101, China

Received:2020-01-13 Online:2020-09-28 Published:2020-09-15

摘要/Abstract

摘要： 目的: 探讨葛根素通过白细胞介素-23(IL-23)/辅助性T 细胞17(Th17)炎症轴对大鼠牙周炎牙槽骨吸收的作用及对骨保护素(OPG)/核因子κB配体(RANKL)/NF-κB受体活化因子(RANK)通路的影响。方法: 构建牙周炎大鼠模型,将SD雄性大鼠随机分为对照组、模型组、葛根素组,每组各20只。葛根素连续给药14 d后,观察各组大鼠牙周组织病理变化及釉牙骨质界至牙槽嵴顶的距离(CEJ-AC);通过HE染色和抗酒石酸酸性磷酸酶(TRAP)染色计数破骨细胞,流式细胞术检测外周血中Th17细胞比例,ELISA法检测血清IL-23、白细胞介素-17(IL-17)表达,通过Western blot法检测牙周组织中OPG、RANKL、RANK蛋白表达。结果: 与对照组相比,模型组和葛根素组CEJ-AC、破骨细胞、Th17细胞比例、血清 IL-23、IL-17水平、RANKL、RANK、RANKL/OPG蛋白水平显著升高(P＜0.05),OPG蛋白水平显著降低(P＜0.05);与模型组相比,葛根素组CEJ-AC、破骨细胞、Th17细胞比例、IL-23、IL-17水平、RANKL、RANK、RANKL/OPG蛋白水平显著降低(P＜0.05),OPG蛋白水平显著升高(P＜0.05)。结论: 葛根素可能通过IL-23/Th17炎症轴上调OPG表达,下调RANKL、RANK蛋白表达,控制牙槽骨吸收,促进牙周炎大鼠牙槽骨的修复和重建。

关键词: 葛根素, 牙周炎, 牙槽骨吸收, 辅助性T细胞17, 白细胞介素23

Abstract: Objective: To investigate the effects of Puerarin on alveolar bone resorption and protein osteoprotegerin (OPG)/nuclear factor κB ligand (RANKL)/NF-κB receptor activating factor (RANK) pathway in rats with periodontitis through interleukin 23 (IL-23)/T helper cell 17 (Th17) inflammatory axis. Methods: The SD male periodontitis rats were set up and randomly divided into control group, model group, and puerarin group, with 20 in each group. After 14 days of Puerarin administration, the pathological changes of periodontal tissues and cementum enamal junction to alveolar crest (CEJ-AC) were observed; osteoclasts were counted by HE staining and tartrate resistant acid phosphatase (TRAP) staining, the proportion of Th17 cells in peripheral blood was detected by flow cytometry, the expressions of IL-23 and interleukin 17 (IL-17) in serum were detected by ELISA, and Western blot was used to detect the expressions of OPG, RANKL and RANK in periodontal tissues. Results: Compared with the control group, the CEJ-AC, osteoclast, Th17 cell ratios, serum IL-23, IL-17 levels, RANKL, RANK, and RANKL/OPG protein levels in the model group and puerarin group were significantly higher (P<0.05), while the level of OPG protein was significantly lower (P<0.05); compared with the model group, the CEJ-AC, osteoclast, Th17 cell ratios, serum IL-23, IL-17 levels, RANKL, RANK, and RANKL/OPG protein levels in puerarin group were significantly lower (P<0.05), while the level of OPG protein was significantly higher (P<0.05). Conclusion: Puerarin may up-regulate OPG expression, down-regulate RANKL and RANK protein expressions through IL-23/Th17 inflammatory axis, so as to control alveolar bone absorption, and promote alveolar bone repair and reconstruction in rats with periodontitis.

Key words: puerarin, periodontitis, alveolar bone absorption, helper T cell 17, interleukin 23

张黎, 刘育菘, 吴芸菲, 符起亚. 基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响[J]. 口腔医学研究, 2020, 36(9): 844-849.

ZHANG Li, LIU Yusong, WU Yunfei, FU Qiya. Effects of Puerarin on Alveolar Bone Resorption and OPG/RANKL/RANK Pathway in Rats with Periodontitis Based on IL-23/Th17 Inflammatory Axis[J]. Journal of Oral Science Research, 2020, 36(9): 844-849.

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基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响

Effects of Puerarin on Alveolar Bone Resorption and OPG/RANKL/RANK Pathway in Rats with Periodontitis Based on IL-23/Th17 Inflammatory Axis

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