Effects of TNF-α on the Proliferation and Odontoblastic as well as Osteogenic Differentiation of SCAP in Vitro.

doi:10.13701/j.cnki.kqyxyj.2016.04.007

Abstract

Abstract: Objective: To investigate the effects of TNF-α on the proliferation and odontoblastic and osteogenic differentiation of stem cells from the apical papilla (SCAP) in vitro. Methods: Stem cells were isolated from the apical papillary by combining enzyme digestion with tissue block method, and identified by immunofluorescence and immunocytochemical method. After treated by TNF-α, the effect on the proliferation and mineralized tooth's ontogenesis ability were detected in SCAP. Results: TNF-α in each density group can stimulate the proliferation of SCAP in vitro, particularly in 10mg/L and 50mg/L (P<0.05). Additionally, in the experimental group, the Alizarin red staining was obvious weaker with smaller mineral node, and the expression of DSP and BSP were down-regulated compared to the control group. Conclusion: TNF-α could promote the proliferation and inhibit the mineralization of SCAP.

CLC Number:

R780.1

YU Li, LI Shu-hui, YUAN Ping, ZHAO Lu, ZHOU Chun-mei, WU Pei-ling.. Effects of TNF-α on the Proliferation and Odontoblastic as well as Osteogenic Differentiation of SCAP in Vitro.[J]. Journal of Oral Science Research, 2016, 32(4): 343-346.

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