Performance of Root Canal Sealing Agent Modified by Polyhexamethylene Guanidine

doi:10.13701/j.cnki.kqyxyj.2016.09.004

Abstract

Abstract: Objective: To prepare and screen the PHMG modified root canal sealing agent which was in accordance with ISO 6876:2001 standard and study its physical properties and antimicrobial activity. Methods: The physical properties of the root canal sealing agents with different PHMG concentrations were measured according to ISO 6876:6876 rules using ZOE paste as control. The ager diffusion test was employed to evaluate the antimicrobial effects of the materials against the following microbes: E. faecalis, C. albicans, E. Coli and S. aureus in vitro. Results: The liquidity, setting time, film thickness and solubility of eleven samples met the ISO 6876:2001 standard. However, only in the group which the concentration of PHMG was 0.8% to 2.0%, the dimensional change met the ISO 6876:2001 standard. The antimicrobial activity of the PHMG modified root canal sealing agent was significantly higher than that of the control group (P＜0.05). Conclusion: Modified PHMG was prepared and met the ISO standard. The root canal sealing agents with the PHMG concentrations between 0.8% and 1.2% showed the best performance.

Key words: PHMG, Root canal sealing agent, Physical properties, Antimicrobial activity

CLC Number:

R781.05

JIA Mei-e , NAI Zheng-gang , LIN Jun , XIE Si-yu , ZHANG Xue-chen , REN Jun , HE Xiang-yi. Performance of Root Canal Sealing Agent Modified by Polyhexamethylene Guanidine[J]. Journal of Oral Science Research, 2016, 32(9): 907-911.

References

[1] Cheung GSP, Chan TK. Long-term survival of primary root canal treatment carried out in a dental teaching hospital [J]. International Endodontic Journal,2003,36(2)∶117-128
[2] Y-L N, Mann V , Rahbaran S , et al. Outcome of primary root canal treatment: systematic review of the literature -- Part 2. Influence of clinical factors [J]. International Endodontic Journal, 2008, 41(1)∶6-31
[3] 彭彬.牙髓病学[M].人民卫生出版社, 2010
[4] Lin LM, Rosenberg PA, Lin J. Do procedural errors cause endodontic treatment failure [J]. Journal of the American Dental Association, 2005, 136(2)∶187-193
[5] Miyagak DC, de Carvalho EM, Robazza CR, etal. In vitro evaluation of the antimicrobial activity of endodontic sealers [J]. Brazilian Oral Research, 2006, 20(4)∶303-306
[6] Nair P N R. On the causes of persistent apical periodontitis: A review [J]. International Endodontic Journal, 2006, 39(4)∶249-281
[7] Tyagi S P, Sinha D J, Garg P, et al. Comparison of antimicrobial efficacy of propolis, Morindacitrifolia, Azadirachtaindica (Neem) and 5% sodium hypochlorite on Candida albicans biofilm formed on tooth substrate: An in-vitro study [J]. Journal of Conservative Dentistry, 2013, 16(6)∶532-535
[8] Hartke A, Giard JC. Survival of Enterococcus faecalis in an Oligotrophic Microcosm: Changes in Morphology [J]. Applied & Environmental Microbiology, 1998, 64(5)∶4238-4245
[9] 刘晓璐,孙汉堂,李鹏,等.感染根管中6种厌氧菌与症状或体征的相关性研究[J].口腔医学研究,2009,25(4)∶451-454
[10] 陈黄琴,范兵,樊明文.经治根管而失败的微生物原因及控制方法[J].国外医学口腔医学分册,2005,32(1)∶58-60
[11] Hasan R, Kazem A Y, Fereshteh J, et al. Antimicrobial Effects of AH26 Sealer/Antibiotic Combinations Against Enterococcus Faecalis [J]. Iranian Endodontic Journal, 2008, 3(4)∶103-108
[12] Bichen L, Yuming Z, Jie Y, et al. Effects of zinc oxide-eugenol and calcium hydroxide/ iodoform on delaying root resorption in primary molars without successors [J]. Dental Materials Journal, 2014, 33(4)∶471-475
[13] Mathias K, Oulé, Richard, et al. Polyhexamethylene guanidine hydrochloride-based disinfectant: a novel tool to fight meticillin-resistant Staphylococcus aureus and nosocomial infections [J]. Journal of Medical Microbiology, 2008, 57(Pt12)∶1523-1528
[14] Andriy, Lysytsya, Mykola, et al. The Antiviral Action of Polyhexamethylene Guanidine Derivatives [J]. Journal of Life Sciences(ISSN1934-7391), 2014(1)∶22-26
[15] 中华人民共和国医药行业标准YY0717—2009,牙科根管封闭材料
[16] Asgary S, Kamrani F A. Antibacterial effects of five different root canal sealing materials [J]. Journal of Oral Science, 2008, 50(4)∶469-474
[17] Saha S, Samadi F, Jaiswal J N, et al. Antimicrobial activity of different endodontic sealers: An in vitro evaluation [J]. Journal of Indian Society of Pedodontics& Preventive Dentistry, 2010, 28(4)∶251-257
[18] Chua E G, Parolia A, Ahlawat P, et al. Antifungal effectiveness of various intracanal medicaments against Candida albicans: an ex-vivo study [J]. Bmc Oral Health, 2014, 14(1)∶1-8
[19] Figdor D, Gulabivala K. Survival against the odds: microbiology of root canals associated with post-treatment disease [J]. Endodontic Topics, 2008, 18(1)∶62-77
[20] Estrela C, Estrada-Bernabé PF, De AD, et al. Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials [J]. Medicina Oral Patologia Oral Y CirugiaBucal, 2011, 16(3)∶e418-424
[21] Leyhausen G, Heil J, Reifferscheid G, et al. Genotoxicity and cytotoxicity of the epoxy resin-based root canal sealer AH plus - Journal of Endodontics [J]. Journal of Endodontics, 1999, 25(2)∶109-113
[22] 林菲,杜德顺.根管封闭剂[J].现代口腔医学杂志,2005,17(1)∶103-106