Obtain the Transgenic Cucumber of the Fusion Gene of Region A of PAc Gene of Streptococcus mutans and B Subunit of Cholera Toxin

doi:10.13701/j.cnki.kqyxyj.2016.09.003

Abstract

Abstract: Objective: To establish cucumber regeneration system, for the fusion gene of region A of pac gene of S.muant and B subunit of cholera toxin so as to provide useful information for further study of edible vaccine against caries. Methods: The plasmid pCAMBIA2301 was used to construct the recombinant plant expression plasmids p2355-PAcA-CTB. The recombinant plasmids were transformed into Agrobacterium tumefaciens EHA105 by electroporation. Cucumber was transformed by co-cultivating leaf discs with Agrobacterium strains harboring p2355-PAcA-CTB. The regenerated kanamycin-resistant plants were analyzed by PCR and Southern blot. Results: The regenerated transgenic cucumber system was obtained successfully. The results of PCR and Southern blot indicated that the fusion gene of streptococcus mutans A region and the CTB B submit (PAcA-ctxB) was integrated into the genomic DNA of the cucumber. Conclusion: Transgenic cucumbers carrying the fusion gene of streptococcus mutans A region and the CTB B submit were obtained.

Key words: Streptococcus mutans, B subunit of cholera toxin, Transgenic cucumber, Agrobacterium tumefaciens

CLC Number:

R780.1

WU Jia-yuan , LIU Jian-guo , MA Xin-rong , HONG Xiang-zhong , WU Zhi-gang , ZHANG Jiang , LIANG Wen-hong. Obtain the Transgenic Cucumber of the Fusion Gene of Region A of PAc Gene of Streptococcus mutans and B Subunit of Cholera Toxin[J]. Journal of Oral Science Research, 2016, 32(9): 902-906.

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