Effects of ALK2 on the Proliferation and Differentiation of Condylar Chondrocytes in Mice

doi:10.13701/j.cnki.kqyxyj.2017.01.002

Abstract

Abstract: Objective: To study the effect of ALK2 on the proliferation and differentiation of mice condylar chondrocytes. Methods: Condylar chondrocytes (MCC) were obtained by enzyme digestion. Immunocytochemistry and real-time PCR were used to identify the isolated cells. SiRNA was used to silence the expression of Alk2 gene in MCC in vitro. Cell proliferation was determined by MTT. The expression of cartilage cell marker genes was analyzed by real-time PCR. Results: Immunocytochemical staining showed strong positive expression of COL I and AGGRECAN, and weak positive expression of COL II. Real-time PCR results showed that the expressions of cartilage related genes such as COL I, COL II, COL X were increased. After silencing ALK2, MTT results showed that cell number was increased and the expression of cartilage related genes was significantly down-regulated. Conclusion: ALK2 could inhibit the proliferation of MCC and promote the chondrogenic differentiation of MCC.

Key words: Condylar, chondrocytes, ALK2, Proliferation, Differentiation

CLC Number:

R782.6

ZHANG Qi, ZHANG Xue, ZHAO Liang, HU Yue, SHI Ce, SUN Hong-chen, HUANG Yang. Effects of ALK2 on the Proliferation and Differentiation of Condylar Chondrocytes in Mice[J]. Journal of Oral Science Research, 2017, 33(1): 6-9.

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