Therapeutic Effects of Mesenchymal Stem Cells Derived from Gingiva on Experimental Autoimmune Encephalomyelitis.

doi:10.13701/j.cnki.kqyxyj.2017.02.001

Abstract

Abstract: Objective: To analyze the therapeutic effects of mesenchymal stem cells derived from gingiva (GMSCs) on experimental autoimmune encephalomyelitis (EAE) and to provide experimental evidence for treatment of EAE using stem cells. Methods: GMSCs and T cells co-culture system were established to analyze the role of GMSCs on T cell proliferation, apoptosis, Th17 and Treg cells differentiation. EAE model was established in mice to explore the therapeutic effects of GMSCs on EAE and the underlying mechanisms. Results: In GMSCs and T cells co-culture system, GMSCs induced T cells apoptosis and inhibit T cells proliferation. GMSCs also inhibited Th17 cells differentiation and promoted Treg cells differentiation. In vivo, GMSCs could ameliorate EAE in mice. Mechanically, GMSCs infusion increased Treg cells differentiation and inhibited Th17 cells differentiation. Conclusion: GMSCs infusion ameliorated EAE in mice by up-regulating Treg cells differentiation and down-regulating Th17 cells differentiation.

Key words: Mesenchymal stem cells derived from gingival , Immunomodulation , Experimental autoimmune encephalomyelitis

CLC Number:

R780.1

YANG Rui-li, YU Ting-ting, ZHOU Yan-heng.. Therapeutic Effects of Mesenchymal Stem Cells Derived from Gingiva on Experimental Autoimmune Encephalomyelitis.[J]. Journal of Oral Science Research, 2017, 33(2): 119-122.

References

[1] 杜令倩,杨丕山,葛少华.人牙龈干细胞的分离鉴定及基质细胞衍生因子-1对其趋化效应的研究[J].华西口腔医学杂志,2016,3(33)∶238-241
[2] Zhang Q, Shi S, Liu Y, et al. Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis [J]. J Immunol, 2009, 4(11)∶7787-7798
[3] Xu X, Chen C, Akiyama K, et al. Gingivae contain neural-crest-and mesoderm dderived mesenchymal stem cells [J]. J Dent Res. 2013, 92(9)∶825-32
[4] Yamaza T, Miura Y, Bi Y, et al. Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodents [J]. PloS one, 2008, 3(7)∶e2615
[5] Chen C, Akiyama K, Yamaza T, et al. Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression [J]. EMBO molecular medicine, 2014, 6(3)∶322-334
[6] Liu Y, Chen C, Liu, S et al. Acetylsalicylic acid treatment improves differentiation and immunomodulation of SHED [J]. Journal of dental research, 2015, 94 (1)∶209-218
[7] Sun L, Akiyama K, Zhang H, et al. Mesenchymal stem cell transplantation reverses multi-organ dysfunction in systemic lupus erythematosus mice and humans. Stem Cells, 2009, 27(6)∶1421-1432
[8] Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses [J]. Blood, 2005, 105(4)∶1815-1822
[9] Zappia E, Casazza S, Pedemonte E, et al. Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy [J]. Blood, 2005, 106(12)∶1755-1761
[10] Zhang X, Koldzic DN, Izikson L, et al. IL-10 is involved in the suppression of experimental autoimmune encephalomyelitis by CD25+CD4+ regulatory T cells [J]. International immunology, 2004, 16(2)∶249-256

[1]	HAN Yi, BAI Yu-hao, HOU Xiao-mei. Glide Path Management of Rotary Nickel-titanium Instruments in Simulated Root Canals [J]. Journal of Oral Science Research, 2018, 34(2): 121-124.
[2]	LIU Xu-yan, WU Zhao-yu, WANG Ran-xu, HE Li-na, PAN Shuang, NIU Yu- mei. Adhesion of Dental Pulp Stem Cells on Zn/polylactic-co-glycolic acid/hydroxylapatite Composite Scaffolds with Different Zinc Contents in Vitro [J]. Journal of Oral Science Research, 2018, 34(2): 169-171.
[3]	SUN Yan-wei, LIU Ya-li, ZHAO Xiao-xue, HOU Fei-fei, ZHANG Xiang-yu. Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans [J]. Journal of Oral Science Research, 2018, 34(1): 27-31.
[4]	YE Zhen, GAO Kai-xian, YIN Xiao-qing. Prevalence of Dental Caries and Related Risk Factors in Primary School Students [J]. Journal of Oral Science Research, 2018, 34(1): 32-34.
[5]	LIANG Xiang-yang, KONG Jing-jing, LI Chun-nian, LI An.. Effects of Different Materials on Remineralization of Demineralized Enamel Caused by Carbonated Beverage. [J]. Journal of Oral Science Research, 2017, 33(12): 1274-1277.
[6]	SUN Dong-liang, YE Tao, LU Qian, YU Shi-bin.. Rewiew on Features of Oral Diseases in Drug Addicted Population. [J]. Journal of Oral Science Research, 2017, 33(12): 1338-1341.
[7]	XU Xiao-chen, WANG Fang, LUO Xin-long, ZHOU Yu, GUO Rui-hua, WU Wen-hui. Optimization of IgY inhibiting Fusobacterium nucleatum proliferation Based on Response Surface Methodology [J]. Journal of Oral Science Research, 2017, 33(10): 1031-1035.
[8]	YIN Zheng-rong, SUN Gui-jun, HU Hui-zhen, WU Tao, HE Xiang-yi. Effect of Sampling Method on Detection of Streptococcus Mutans [J]. Journal of Oral Science Research, 2017, 33(10): 1056-1059.
[9]	FENG Xue-feng, CHENG Jian-feng, ZHAO He, ZHAO Xin-hao, LIN Zhao-hong. Investigation and Countermeasures of Health Self-Management Ability of the Oral Medical Students [J]. Journal of Oral Science Research, 2017, 33(10): 1071-1073.
[10]	CHEN Qi, REN Ting, WANG Mei, WANG Ya-ke. Application and Evaluation of Localized Management of Administration in Dental Clinical Practice [J]. Journal of Oral Science Research, 2017, 33(10): 1106-1109.
[11]	WU Wen-zhen, WANG Kai-juan, WANG Jia-wei. Review on Correlation Between Long Non-coding RNAs and Cell Differentiation [J]. Journal of Oral Science Research, 2017, 33(10): 1127-1130.
[12]	DONG Wei, CHEN Rui, YAN Quan, BAO Guang-jie. Effects of Three Plant Extracts on Cariogenic Tolerance of Streptococcus Mutans [J]. Journal of Oral Science Research, 2017, 33(9): 943-945.
[13]	ZHAO Yuan, WANG Shu, SHAO Bo, GAO Ying. Effects of PTEN on Proliferation and Apoptosis of Periodontal Ligament Cells Induced by Hydrogen Peroxide. [J]. Journal of Oral Science Research, 2017, 33(8): 889-892.
[14]	CHEN Zhi-hui, LV Li-juan, ZHANG Liang, YE Ning. Research Progress of Relationship Between Oral Organisms and Adverse Pregnancy Outcomes. [J]. Journal of Oral Science Research, 2017, 33(8): 905-908.
[15]	LI Rong, WANG Yi-ning,MA Xiao.. Expression of TRPAl in Rats Dental Pulp Induced by Dentin Hypersensitivity. [J]. Journal of Oral Science Research, 2017, 33(7): 689-692.