Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance.

doi:10.13701/j.cnki.kqyxyj.2017.04.004

Abstract

Abstract: Objective: To investigate the effect of occlusal intervention on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in TMJ synovium of rats caused by increased anterior guidance. Methods: Metal crowns increased 30 degree on palatal side were cemented to the maxillary incisors of rat to build the model of anterior guidance increased group (C). The intervention group was built by increased the anterior guidance of rat first, then the occlusal intervention respectively started on the day 3, 7, 14, and 21 to build the I-3d, I-7d, I-14d, and I-21d intervention group. All rats were sacrificed on day 28. TMJ joints were extracted by one side. HE staining was processed to observe the histological changes of TMJ synovium. IHC and real-time PCR analysis were evaluated to detect the levels of COX-2 and iNOS in TMJ synovium. Results: The mRNA of iNOS and COX-2 barely expressed in the intervention groups (I) compared with the anterior guidance increased group (C) (P<0.05). iNOS and COX-2 were scarcely expressed in the group I-3d. Conclusion: On the basis of increasing the anterior guidance inclination of rat, the early intervention would lead to much less expression of iNOS and COX-2 in TMJ synovium of rat.

Key words: Anterior guidance , Occlusal intervention, Temporomandibular joint synovium, Cyclooxygenase-2Inducible nitric oxide synthase

CLC Number:

R782.6

LI Yu-xuan, YAO Tian-hua, BAI Le-kang, TAN Wen-hong, HAN Hui-fang, ZHAI Tong, XIAO Min.. Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance.[J]. Journal of Oral Science Research, 2017, 33(4): 362-366.

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