Preparation and Cytocompatibility of Adult and Fetal Bovine Beta-TCP Calcined Bone Blocks.

doi:10.13701/j.cnki.kqyxyj.2017.05.003

Abstract

Abstract: Objective: To prepare a kind of bone graft material with higher purity on β-TCP, better properties and perfect cytocompatibility according to the representation and biocompatibility of adult and fetal bovine's β-TCP calcined bone blocks prepared with NP and MAP. Methods: XRD diffraction analysis, EDX spectrum analysis, electron microscope scanning, Micro-CT scanning, and universal material testing machine were used to compare the properties of two bone blocks. The in vitro cytotoxicity-test and cell-to-material adhesion experiment were used to explore the cytocompatibility. Results: The main ingredients of calcined bone blocks prepared with MAP were similar to pure phase β-TCP, and their Ca/P ratio were close to 1.47. The fetal bovine calcined bone blocks' porosity, compressive strength, and bending strength were higher than those of adult cattle (P<0.05). Two kinds of calcined bone blocks' cytotoxicity were 0 levels, but cell adhesion and proliferation rate on fetal bovine bone blocks were higher than those on adult cattle calcined bone blocks. Conclusion: The fetal bovine calcined bone blocks prepared by MAP have better mechanical properties and cytocompatibility, and they are more suitable to apply for bone defect repair in the non-bearing area.

CLC Number:

R780.1

ZHOU Jian-ye, MA Rui, SHI Wen, YU Zhan-hai, LI Zhi-qiang, ZHANG Xiao-feng.. Preparation and Cytocompatibility of Adult and Fetal Bovine Beta-TCP Calcined Bone Blocks.[J]. Journal of Oral Science Research, 2017, 33(5): 475-478.

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