Effects of Irradiation on IK1 and BK Channels in Rat Submandibular Glands.

doi:10.13701/j.cnki.kqyxyj.2017.08.007

Abstract

Abstract: Objective: To explore the mechanism of irradiation-induced salivary glands hypofunction by investigating the change of IK1 and BK channels electric current after irradiation in rat submandibular gland. Methods: Male Wistar rats, weight 180-220g, were randomly divided into control group and irradiation group. The bilaterally submandibular glands of irradiation group were irradiated with a single dose of 15Gy. The control rats were only anesthesia but no irradiated. On the pre-IR, post-IR 3rd day, 15th day and 40th day, respectively, the macroscopic K⁺ currents change of IK1 and BK channels in acinar cells of submandibular glands were recorded with whole-cell patch clamp experiments. Results: At +80 mV, in compared with control group, the current density of BK channel and IK1 channel of 3 day group was not significant different, and 15 day group reduced 9.7% and 12.8%, respectively (P<0.05), and 40 day group reduced 13.8% and 17.1%, respectively (P<0.01). Conclusion: Irradiation can reduce the K⁺ current densities of BK channel and IK1 channel in rat submandibular gland, which may be one reason for radiation-induced salivary glands hypofunction.

Key words: Xerostomia , Potassium channel , Radiation, Submandibular , Patch clamp

CLC Number:

R781.7

ZHU Hong-hua, GONG Bai-juan, SONG Zi-qi, JU Hao, FENG Jing, LI Zhi-min, SUN Hong-chen.. Effects of Irradiation on IK1 and BK Channels in Rat Submandibular Glands.[J]. Journal of Oral Science Research, 2017, 33(8): 828-831.

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