Effect of Early Intervetion by Botulinum Toxin Type A on Mandible Growth.

doi:10.13701/j.cnki.kqyxyj.2017.08.009

Abstract

Abstract: Objective:To evaluate the effects of botulinum toxin type A on mandible overdevelopment in animal model. Methods: Three-week-old Sprague-Dawley female rats (n=35) were divided into seven groups: five rats in group 1 served as normal control and received no treatment, five rats in group 2 as negative control and their bilateral masseters were injected with peanut oil (solvent oil of Nandrolone Decanoate) at the fourth week, and the rest 25 rats were divided into group 3 to grouop 7. They were received injection of Nandrolone Decanoate in bilateral masseters every week and four groups, group 4 to group 7, were injected with botulinum toxin type A in bilateral masseters at the third to the seventh week, respectively. The mandibles were harvested at the fifteenth week and scanned by Mirco-CT. And the data generated were imported into Mimics to reconstruct 3D model of mandibles and analyzed. Results: All indexes in group 2 were similar to those in group 1. Compared with group 1 and group 2, the mandible indexes in group 3 significantly increased. The indexes in group 5, 6, and 7 were similar to those in group 1. Compared with group 5, 6 and 7, the length and height of mandible in group 3 increased. The length and height of mandible decreased in BTX-A treated group. Conclusion: Local injection of nandrolone decanoate into rats’ bilateral masseter can induce mandible overdevelopment in pubertal rats, which can be prevented by local injection of BTX-A into rats’ masseter. The preventive efficacy peaks at the fifth week post treatment by nandrolone decanoate.

Key words: Botulinum toxin type, A Nandrolone, Decanoate, Mandible growth

CLC Number:

R739.8

PIAO Zheng-guo, ZOU Rui, LIAO Ting, YAN Jing.. Effect of Early Intervetion by Botulinum Toxin Type A on Mandible Growth.[J]. Journal of Oral Science Research, 2017, 33(8): 837-840.

References

[1] Tsai CY, Shyr YM, Chiu WC, et al. Bone changes in the mandible following botulinum neurotoxin injections [J]. The European Journal of Orthodontics, 2011, 33(2)∶132-138
[2] Ulgen M, Baran S, Kaya H, et al. The influence of the masticatory hypofunction on the craniofacial growth and development in rats [J]. Am J Orthod Dentofacial Orthop, 1997, 111(2)∶189-198
[3] 张永富,居来提·吐尔逊,买买提吐逊·吐尔地. 交互式医学图像控制系统(MIMICS)在下颌骨肿瘤切除及缺损重建中的应用[J].口腔医学研究,2016,10(18)∶1074-1078
[4] Bas B, Ozan B, Muglali M, et al. Treatment of masseteric hypertrophy with botulinum toxin: a report of two cases [J]. Med Oral Patol Oral Cir Bucal, 2010, 15(4)∶e649-e652
[5] Rafferty K L, Liu Z J, Ye W, et al. Botulinum toxin in masticatory muscles: Short- and long-term effects on muscle, bone, and craniofacial function in adult rabbits [J]. Bone, 2012, 50(3)∶651-662
[6] Kim J Y, Kim S T, Cho S W, et al. Growth effects of botulinum toxin type A injected into masseter muscle on a developing rat mandible [J]. Oral Dis, 2008, 14(7)∶626-632
[7] Matthys T, Ho Dang HA, Rafferty KL, et al. Bone and cartilage changes in rabbit mandibular condyles after 1 injection of botulinum toxin [J]. American Journal of Orthodontics and Dentofacial Orthopedics, 2015, 148(6)∶999-1009
[8] Rando C, Hillson S, Antoine D. Changes in mandibular dimensions during the mediaeval to post-mediaeval transition in London: A possible response to decreased masticatory load [J]. Archives of Oral Biology, 2014, 59∶73-81
[9] Navarrete A, Rafferty K, Liu ZJ. Botulinum neurotoxin type A in the masseter muscle: Effects on incisor eruption in rabbits [J]. American Journal of Orthodontics and Dentofacial Orthopedics, 2013, 143(4)∶499-506
[10] Nakamura, Aya Z, Jorge L. Influence of malocclusion on the development of masticatory function and mandibular growth [J]. The Angle Orthodontist, 2013, 83(5)∶749-757
[11] Chen Z, Chen Z, Zhao N, et al. An Animal Model for Inducing Deviation of the Mandible [J]. J Oral Maxillofac Surg, 2015, 73(11)∶2207-2218
[12] Kwon TG, Park HS, Lee SH, et al. Influence of unilateral masseter muscle atrophy on craniofacial morphology in growing rabbits [J]. J Oral Maxillofac Surg, 2007, 65(8)∶1530-1537
[13] Park C, Park K, Kim J. Growth effects of botulinum toxin type A injected unilaterally into the masseter muscle of developing rats [J]. J Zhejiang Univ Sci B, 2015, 16(1)∶46-51
[14] Moss M L, Rankow R M. The role of the functional matrix in mandibular growth [J]. Angle Orthod, 1968, 38(2)∶95-103

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