Effects of Regenerated Fluorapatite Crystal Film on Early Adhesion and Biofilm Growth of Streptococcus Mutans

doi:10.13701/j.cnki.kqyxyj.2021.03.006

Abstract

Abstract: Objective: To investigate the effects of fluorapatite crystal film (FACF) on early adhesion and biofilm growth of Streptococcus mutans (S. mutans). Methods: Under near-physiological conditions (37℃, 1 atm, pH 6.0-7.4), FACF was regenerated on the surface of exposed dentin. The dentin, hydroxyapatite (HA), and FA were prepared as the control groups. SEM was used to observe the surface morphology of the samples. The adhesion of S. mutans on the surface of dentin and FACF was observed with confocal laser scanning microscope (CLSM) and SEM. The S. mutans biofilm was cultured on the surfaces of HA, FA, dentin, and FACF, which was observed by CLSM. The amount of biofilm was calculated. Results: The thickness of FACF was about 5-10 μm. The crystal was standard six-prism shape with a diameter of 0.5-1 μm and arranged closely and orderly. The adhesion amount of S. mutans of FACF was significantly less than that of dentin (P<0.05), and the amount of biofilm per unit area on FACF surfaces was significantly lower than that of HA, FA, and dentin surfaces (P<0.05). FACF could prevent S. mutans from growing into dentin tubules. Conclusion: Under near physiological conditions, a layer of dense and orderly FA crystal film can be regenerated on the dentin surface. The crystal structure of this layer can reduce the adhesion of S. mutans and formation of biofilm, and act as a physical barrier to the growth of S. mutans into dentin tubules.

Key words: dentin, fluorapatite crystal film, Streptococcus mutans, adhesion, barrier effect

YANG Suming, LIU Shize, CHEN Haifeng, WANG Xiaoyan. Effects of Regenerated Fluorapatite Crystal Film on Early Adhesion and Biofilm Growth of Streptococcus Mutans[J]. Journal of Oral Science Research, 2021, 37(3): 208-212.

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