Effect of Piezo1 Channel on Expression of IL-1β, IL-6, and IL-17 in Periodontal Tissue of Pressure Side During Tooth Movement

doi:10.13701/j.cnki.kqyxyj.2023.02.009

Abstract

Abstract: Objective: To investigate the distribution of Piezo1 in the pressure side of periodontal tissue and its effect on the expression of IL-1β, IL-6, and IL-17 by using the orthodontic tooth movement model in rats, and to further understand the role of Piezo1 in the reconstruction of periodontal tissue during orthodontic process. Methods: Sixty male SD rats aged 6-8 weeks were randomly divided into group A (control group), group B (force group), and group C (force + inhibitor group), which were further divided into five subgroups according to 1, 3, 5, 7, and 14 days. The teeth movement model was constructed in group B and group C, and Piezo1 inhibitor was locally injected into group C. The rats were sacrificed at the specified time point and the tooth movement distance was measured. HE staining was used to observe the morphological changes of periodontal tissue on the pressure side, and immunohistochemical staining was used to observe the expression of Piezo1, IL-1β, IL-6, and IL-17 in the periodontal tissue on the pressure side. Results: The displacement distances of teeth in group B and group C increased with time, and the morphological changes of periodontal tissue were obvious. The expressions of Piezo1, IL-1β, IL-6, and IL-17 in group B were significantly higher than those in group A (P<0.05). No significant difference was found between group C and group B except Piezo1 (P>0.05). The expression levels of IL-1β, IL-6, and IL-17 were lower at day 3, 5, and 7 (P<0.05), but still higher than those of group A (P<0.05). Conclusion: Piezo1 is involved in the movement of orthodontic teeth, which affects the expression of inflammatory factors and participates in the reconstruction of periodontal tissue of pressure side during orthodontic tooth movement.

Key words: orthodontic tooth movement, Piezo1, interleukins-1β, interleukins-6, interleukins-17

ZHANG Man, ZHANG Miaomiao, LI Yi, WANG He, ZHANG Yanyun. Effect of Piezo1 Channel on Expression of IL-1β, IL-6, and IL-17 in Periodontal Tissue of Pressure Side During Tooth Movement[J]. Journal of Oral Science Research, 2023, 39(2): 135-140.

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