Selenomethionine Inhibited Expression of iNOS and COX-2 in P.g-LPS Induced RAW264.7 Cells through NF-κB and MAPK Pathways

doi:10.13701/j.cnki.kqyxyj.2023.03.010

Abstract

Abstract: Objective: To demonstrate the effects and mechanism of selenomethionine (SeMet) on porphyromonas gingivalis lipopolysaccharide (P.g-LPS) induced RAW264.7. Methods: P.g-LPS was used to stimulate the RAW264.7 cell line. CCK-8 was used to demonstrate the cell viability of RAW264.7 cells induced by SeMet on different concentration. After RAW264.7 was pretreated with SeMet (10,25,50μmol/L) for 1 h and then cultured with or without 1 μg/mL P.g-LPS for 24h, RT-PCR was used to clarify the gene expression of iNOS and COX-2, ELISA assay was used detect the protein secretion, and Western blot was used to determine the relative protein expression of NF-κB and MAPK signaling pathway. Results: SeMet lower than 50 μmol/L had no significant influence on RAW264.7 cell viability. After the RAW264.7 cells were pretreated with SeMet, the relative gene expression and protein secretion of iNOS and COX-2 were significantly inhibited by SeMet. The higher the concentration, the greater the inhibition. The mechanism was via inhibiting nuclear transcription of NF-κB p65 and phosphorylation of p38, ERK, and JNK of MAPK signaling pathway. The inhibiting effects were concentration-dependent. Conclusion: SeMet inhibited the expression of iNOS and COX-2 on RAW264.7 induced by P.g-LPS. The mechanism was related to inhibition of nuclear transcription of NF-κB p65 and phosphorylation of p38, ERK, and JNK of MAPK signaling pathway.

Key words: selenomethionine, Porphyromonas gingivalis lipopolysaccharide, macrophage, NF-κB signaling pathway

ZHANG Fu, ZHOU Ping, XIANG Feng, WANG Chunyan, LI Lei. Selenomethionine Inhibited Expression of iNOS and COX-2 in P.g-LPS Induced RAW264.7 Cells through NF-κB and MAPK Pathways[J]. Journal of Oral Science Research, 2023, 39(3): 236-241.

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