RAPTOR Affects Migration, Invasion, and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p

doi:10.13701/j.cnki.kqyxyj.2024.05.010

Abstract

Abstract: Objective: To investigate the effect of regulatory-associated protein of mTOR (RAPTOR) on the migration, invasion, and proliferation of oral squamous cell carcinoma (OSCC). Methods: The TCGA database was used to identify differentially expressed mRNA in head and neck squamous cell carcinoma (HNSCC) tissues and adjacent tissues. Western blot assay detected RAPTOR expression in HOEC and OSCC cell lines. Cell migration ability was detected by the wound healing experiment. Transwell assay and EdU were used to detect the cell invasion and cell proliferation ability. Bioinformatics websites were used to predict microRNA binding to RAPTOR. Functional tests were conducted to determine if miR-485-5p could impact cell migration, invasion, and proliferation by targeting RAPTOR. Results: RAPTOR expression was higher in HNSCC tissues compared to adjacent tissues. Wound healing, Transwell, and EdU experiments showed that RAPTOR promoted the migration, invasion, and proliferation of OSCC cells. Up-regulation of miR-485-5p can reverse the promoting effect of RAPTOR on the migration, invasion, and proliferation of CAL27 cells. Conclusion: miR-485-5p inhibits OSCC cell migration, invasion, and proliferation by targeting RAPTOR.

Key words: RAPTOR, miR-485-5p, oral squamous cell carcinoma, migration, invasion, proliferation

CHEN Jiawen, DING Xiao, LUAN Kefeng, WANG Gaojun, LI Hongli, HU Wenting, SUN Xuehui. RAPTOR Affects Migration, Invasion, and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p[J]. Journal of Oral Science Research, 2024, 40(5): 429-435.

References

[1] Siegel RL, Miller KD, Wagle NS, et al. Cancer statistics, 2023 [J]. CA Cancer J Clin, 2023, 73(1): 17-48.
[2] Chen SH, Hsiao SY, Chang KY, et al. New insights into oral squamous cell carcinoma: From clinical aspects to molecular tumorigenesis [J]. Int J Mol Sci, 2021, 22(5):2252.
[3] Johnson DE, Burtness B, Leemans CR, et al. Head and neck squamous cell carcinoma [J]. Nat Rev Dis Primers, 2020, 6(1): 92.
[4] Ferlay J, Colombet M, Soerjomataram I, et al. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods [J]. Int J Cancer, 2019, 144(8): 1941-1953.
[5] Ling Z, Cheng B, Tao X. Epithelial-to-mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities [J]. Int J Cancer, 2021, 148(7): 1548-1561.
[6] Saxton RA, Sabatini DM. mTOR signaling in growth, metabolism, and disease [J]. Cell, 2017, 168(6): 960-976.
[7] Khan KH, Yap TA, Yan L, et al. Targeting the PI3K-AKT-mTOR signaling network in cancer [J]. Chin J Cancer, 2013, 32(5): 253-265.
[8] Li X, Sun C, Chen J, et al. Suppression of FAM83D inhibits glioma proliferation, invasion and migration by regulating the AKT/mTOR signaling pathway [J]. Transl Oncol, 2022, 22: 101454.
[9] Shi Z, Liu R, Lu Q, et al. UBE2O promotes hepatocellular carcinoma cell proliferation and invasion by regulating the AMPKα2/mTOR pathway [J]. Int J Med Sci, 2021, 18(16): 3749-3758.
[10] Liao H, Zhang L, Lu S, et al. KIFC3 promotes proliferation, migration, and invasion in colorectal cancer via PI3K/AKT/mTOR signaling pathway [J]. Front Genet, 2022, 13: 848926.
[11] Tan Y, Wang Z, Xu M, et al. Oral squamous cell carcinomas: state of the field and emerging directions [J]. Int J Oral Sci, 2023, 15(1): 44.
[12] Kondo S, Hirakawa H, Ikegami T, et al. Raptor and rictor expression in patients with human papillomavirus-related oropharyngeal squamous cell carcinoma [J]. BMC Cancer, 2021, 21(1): 87.
[13] Zhu Y, Wang S, Niu P, et al. Raptor couples mTORC1 and ERK1/2 inhibition by cardamonin with oxidative stress induction in ovarian cancer cells [J]. PeerJ, 2023, 11: e15498.
[14] Bhat GR, Hyole RG, Li J. Head and neck cancer: Current challenges and future perspectives [J]. Adv Cancer Res, 2021, 152: 67-102.
[15] Yang H, Jiang X, Li B, et al. Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40 [J]. Nature, 2017, 552(7685): 368-373.
[16] Bandres E, Agirre X, Ramirez N, et al. MicroRNAs as cancer players: potential clinical and biological effects [J]. DNA Cell Biol, 2007, 26(5): 273-282.
[17] Bi G, Liang J, Zhao M, et al. miR-6077 promotes cisplatin/pemetrexed resistance in lung adenocarcinoma via CDKN1A/cell cycle arrest and KEAP1/ferroptosis pathways [J]. Mol Ther Nucleic Acids, 2022, 28: 366-386.
[18] Toda H, Seki N, Kurozumi S, et al. RNA-sequence-based microRNA expression signature in breast cancer: tumor-suppressive miR-101-5p regulates molecular pathogenesis [J]. Mol Oncol, 2020, 14(2): 426-446.
[19] Sun S, Wang J, Liu J, et al. MiR-302b suppresses tumor metastasis by targeting frizzled 6 in OSCC [J]. J Dent Res, 2021, 100(7): 739-745.
[20] Wang Y, Jia RZ, Diao S, et al. miRNA-101 targets TGF-βR1 to retard the progression of oral squamous cell carcinoma [J]. Oncol Res, 2020, 28(2): 203-212.
[21] Wang X, Zhou X, Zeng F, et al. miR-485-5p inhibits the progression of breast cancer cells by negatively regulating MUC1 [J]. Breast Cancer, 2020, 27(4): 765-775.