Effects of Escherichia Coli Lipopolysaccharide on Biological Behaviors of Rat Dental Follicle Stem Cells

doi:10.13701/j.cnki.kqyxyj.2023.06.013

Abstract

Abstract: Objective: To investigate the effects of Escherichia coli lipopolysaccharide (E. coli LPS) on biological behaviors of rat dental follicle stem cells (rDFSCs). Methods: rDFSCs were cultured and identified, and then were treated with different concentrations of E. coli LPS (0, 1, 10 μg/mL). The mRNA levels of pro-inflammatory genes were measured by quantitative real-time fluorescence polymerase chain reaction (qRT-PCR). The effects of LPS on proliferation and migration ability of rDFSCs were assessed by CCK-8 and scratch test plus transwell assay, respectively. The osteogenic capability of rDFSCs was detected by alkaline phosphatase (ALP) staining and alizarin red staining, and their osteogenic-related gene mRNA levels were measured by qRT-PCR. Results: The gene expressions of IL-6, IL-1 β, and TNF-α were significantly upregulated under 1 or 10 μg/mL LPS treatment (P＜0.05). LPS at 1 μg/mL significantly promoted the proliferation and migration of rDFSCs (P＜0.05), while a higher concentration (10 μg/mL) did not show any improvement. ALP staining intensity and the number of mineralized nodules decreased, and the expressions of osteogenic markers RUNX2, COL1, and OPN were inhibited in LPS-treated group (P＜0.05).Conclusion: The osteogenic ability of rDFSCs was significantly inhibited under inflammatory microenvironment simulated by E.coli LPS, and LPS promoted the proliferation and migration of rDFSCs at low concentration.

Key words: lipopolysaccharide, dental follicle stem cells, cell proliferation, cell migration, osteogenic differentiation

JIANG Zhen, YAN Yanhong, HAN Xue, JIANG Beizhan. Effects of Escherichia Coli Lipopolysaccharide on Biological Behaviors of Rat Dental Follicle Stem Cells[J]. Journal of Oral Science Research, 2023, 39(6): 534-540.

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