Effect of Polygonatum Sibiricum Polysaccharides on Osteogenic Differentiation of Adipose Derived Stem Cells in Osteoporosis Mice

doi:10.13701/j.cnki.kqyxyj.2022.02.013

Abstract

Abstract: Objective: To study the effect of polygonatum sibiricum polysaccharides (PSP) on osteogenic differentiation of adipose derived stem cells in osteoporosis mice (OP-ASCs). Methods: Osteoporosis mouse model was established. OP-ASCs were isolated and cultured in vitro. Cell proliferation was detected by CCK-8 method after using different concentrations of PSP to intervene in OP-ASCs. Osteogenic capacity was analyzed by western-blot, real-time PCR, and ALP staining at day 3 and 5 after osteogenic differentiation in PSP-intervened OP-ASCs. Results: When the concentration of PSP was 5, 10, 25, and 50 mg/L, the proliferation of OP-ASCs was not significantly affected on day 3 and 5 (P>0.05). Western-blot and real-time PCR results showed that Runx2, OPN, β-catenin, P-GSK-3β protein and Runx2, Opn, β-catenin gene expression in the treatment group were significantly higher than those in the control group (P<0.05). ALP staining showed that the osteogenic effect was significantly higher than the control group. Conclusion: PSP can enhance osteogenic differentiation of OP-ASCs, which may be achieved by up-regulating Wnt/β-catenin signaling pathway.

Key words: polygonatum sibiricum polysaccharides, osteoporosis, adipose-derived stem cells, osteogenic differentiation

LU Shi, HE Qingming, LOU Fangzhi, PENG Shuanglin, GAO Yujin, XIAO Jingang. Effect of Polygonatum Sibiricum Polysaccharides on Osteogenic Differentiation of Adipose Derived Stem Cells in Osteoporosis Mice[J]. Journal of Oral Science Research, 2022, 38(2): 157-163.

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