Effects of Soluble Epoxide Hydrolase Inhibitor on Proliferation and Osteogenic Differentiation of Stem Cells from Apical Papilla in Inflammatory Environment

doi:10.13701/j.cnki.kqyxyj.2022.02.014

Abstract

Abstract: Objective: To investigate the effect of TPPU on proliferation and differentiation of hSCAPs in the inflammatory microenvironment. Methods: hSCAPs were cultured and identified by flow cytometry and alizarin red staining. The hSCAPs were treated with 1 mg/mL lipopolysaccharide (LPS) to simulate the inflammatory microenvironment, and then treated with TPPU. The CCK-8 method was used to study whether TPPU had an effect on the proliferation ability of hSCAPs on the first 1, 3, 5, and 7 days. After 24 hours cultivation, the expressions of inflammation-related factors were detected by qRT-PCR. On the day 7 and 12, the expression levels of genes related to osteogenic differentiation were detected by qRT-PCR. On the day 7, ALP activity was analyzed by staining. On day 21, the formation of mineralized nodules was observed by alizarin red staining. Results: hSCAPs were confirmed by flow cytometry and alizarin red staining. CCK-8 results showed that there was no significant difference in OD value between hSCAPs. The expression of IL-1β and IL-6 of hSCAPs induced by LPS was significantly up-regulated compared with the vehicle group (P<0.05). Compared with the LPS group, the expression of IL-1β and IL6 in LPS+TPPU group was significantly down-regulated (P<0.05), but the expression levels of osteogenic genes in LPS+TPPU group were significantly up-regulated (P<0.05). ALP staining and alizarin red staining showed that the TPPU treated groups had deeper staining than LPS groups. Conclusion: TPPU can inhibit the expression of inflammatory factors in inflammatory microenvironment. TPPU may not promote the proliferation, but promote the tooth/osteogenic differentiation of hSCAP in inflammatory microenvironment.

Key words: soluble epoxide hydrolase inhibitor, human stem cells from apical papilla, lipopolysaccharide, proliferation, differentiation

DANG Haixia, WANG Fu, CHEN Lan, WANG Yulin, ZHOU Zhiqiang. Effects of Soluble Epoxide Hydrolase Inhibitor on Proliferation and Osteogenic Differentiation of Stem Cells from Apical Papilla in Inflammatory Environment[J]. Journal of Oral Science Research, 2022, 38(2): 164-168.

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