SIRT1 Enhances Osteogenesis in Stem Cells from Human Exfoliated Deciduous Teeth via Downregulation of Peroxisome Proliferator-Activated Receptor

doi:10.13701/j.cnki.kqyxyj.2022.02.015

Abstract

Abstract: Objective: To identify the role of SIRT1 on osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Methods: A defined concentration of SIRT1 activator (SRT1720) and inhibitor (EX527) is 0.5 μmol/L. Then SRT1720 and EX527 were added to the experiment groups for preconditioning 3 days. We investigated the effects of SIRT1 on the osteogenic differentiation of SHEDs. Expression of osteoblastic differentiation markers was assessed by RT-PCR. Results: Activation of SIRT1 using SRT1720 increased the expression of osteoblastic differentiation markers, as assessed by alkaline phosphatase, Runt-related transcription factor-2, osteopontin, collagen type Iα1, and osteocalcin. In contrast, inhibition of SIRT1 using EX527 suppressed the expression of above-mentioned factors. Conclusion: SIRT1 is a key regulator of differentiation of stem cells from human exfoliated deciduous teeth. SIRT1 also regulated the expression of PPAR-γ. The SIRT1/PPAR-γ pathway may be crucial for enhancement of osteogenesis.

Key words: SIRT1, SHEDs, PPAR-γ, osteogenic differentiation

CAO Wei, LIU Mengjia, ZHANG Qingbin. SIRT1 Enhances Osteogenesis in Stem Cells from Human Exfoliated Deciduous Teeth via Downregulation of Peroxisome Proliferator-Activated Receptor[J]. Journal of Oral Science Research, 2022, 38(2): 169-175.

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