Proteomic Analysis of Inflammation-related Differential Proteins in Human Dental Pulp Stem Cells Induced by Porphyromonas Gingivalis Lipopolysaccharide

doi:10.13701/j.cnki.kqyxyj.2022.08.008

Abstract

Abstract: Objective: To investigate the expression of inflammation-related differential proteins in human dental pulp stem cells (DPSCs) induced by Porphyromonas gingivalis lipopolysaccharide (P.gingivalis LPS) by proteomics. Methods: Human DPSCs were isolated, cultured, and identified in vitro. Ten μg/mL P.gingivalis LPS was used to stimulate DPSCs to establish an inflammatory model. The expressions of inflammatory factors interleukin-6 (IL-6) and IL-8 were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). The differentially expressed proteins related to inflammation induced by P.gingivalis LPS were screened by proteomics, and the functional enrichment was analyzed by KEGG, GO, and PPI. Results: P.gingivalis LPS stimulated the expression of inflammatory factors IL-6 and IL-8 in DPSCs. Compared with the control group, 277 differentially expressed proteins (DEPs) in DPSCs were changed significantly in the inflammatory microenvironment. Functional enrichment analysis showed that DEPs were involved in regulating multiple inflammatory or damage repair related signal pathways, such as neutrophil mediated immune process, tumor necrosis factor-mediated signal pathway, apoptosis signal pathway, Wnt pathway, TGF-β biological processes such as signal pathway, pentose phosphate pathway, and HIF-1 signal pathway. Conclusion: P.gingivalis LPS can induce an inflammatory response in DPSCs. This process involves the relationship between multiple proteins and multiple signal pathways, which lays a foundation for exploring the pathogenesis of pulpitis and the study of clinical diagnostic markers.

Key words: porphyromonas gingivalis, lipopolysaccharide, dental pulp stem cells, proteomics, bioinformatics

XIE Yuandong, LI Zehua, LIU Xiaoli, HU Ling, ZHAN Luoning, LI Yi. Proteomic Analysis of Inflammation-related Differential Proteins in Human Dental Pulp Stem Cells Induced by Porphyromonas Gingivalis Lipopolysaccharide[J]. Journal of Oral Science Research, 2022, 38(8): 736-741.

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