Effect of Resolvin E1 on Neutrophil Apoptosis in Pulpitis

doi:10.13701/j.cnki.kqyxyj.2022.08.009

Abstract

Abstract: Objective: To investigate the effect and mechanism of resolvin E1 (RvE1) on apoptosis of neutrophils in pulpitis. Methods: SD rats were used to establish the pulpitis model and RvE1 was used for pulp capping. Samples were collected 1, 3, and 7 days after surgery. HE staining was performed to detect inflammatory infiltration, and the expression of inflammatory factors were detected by immunohistochemistry. CD11b and Tunel co-staining were used to detect the neutrophil apoptosis rate. Human neutrophils were isolated and cultured with heat-inactived Streptococcus mutans (S.mutan) or lipopolysaccharide after pretreated with RvE1. Samples were collected 6, 12, and 24 hours after stimulation. Annexin V/PI was used to detect the apoptosis rate and qPCR was used to detect mRNA expression of apoptosis related factors. Results: In vivo, RvE1 treatment significantly reduced the pulpitis infiltration and expressions of TNF-α and IL-1β, and increased the neutrophil apoptosis rate. In vitro, RvE1 treatment increased the apoptosis rate of neutrophil after S.mutan stimulation. QPCR results showed that the expression of pro-survival gene Akt and Mcl-1 decreased, and the expression of pro-apoptotic gene Bax increased. Conclusion: RvE1 regulates the expression of Akt, Mcl-1, and Bax, enhances the apoptosis of neutrophils after bacteria stimulation, and promotes the regression of pulpitis.

Key words: neutrophil, apoptosis, resolution, pulplitis

PAN Liangliang, ZHANG Qi. Effect of Resolvin E1 on Neutrophil Apoptosis in Pulpitis[J]. Journal of Oral Science Research, 2022, 38(8): 742-746.

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