Effect of Soluble Epoxide Hydrolase Inhibitor on Migration and Odontogenic Differentiation of Human Dental Pulp Stem Cells Exposed to HEMA

doi:10.13701/j.cnki.kqyxyj.2024.02.014

Abstract

Abstract: Objective: To investigate the effect of soluble epoxide hydrolase inhibitor (TPPU) on the migration and odontogenic differentiation of human dental pulp stem cells (hDPSCs) exposed to 2-hydroxyethyl methacrylate (HEMA). Methods: hDPSCs were isolated and extracted, and then identified by alizarin red staining and flow cytometry. hDPSCs without drug culture were used as the control group, and HEMA and HEMA+TPPU were used as the experimental group. hDPSCs activity was detected by CCK-8. hDPSCs migration was detected by Scratch test. ALP activity was detected and be quantified by alkaline phosphatase staining and quantitative test kit. Real-time quantitative fluorescent PCR (RT-qPCR) was used to detect the relative expression of Runx2, DMP-1, and DSPP related to odontogenic differentiation. Alizarin red staining was used to detect the formation of mineralized nodules. Results: The cell activity of HEMA group was lower than that of control group (P<0.05), and the cell activity of HEMA+TPPU group was higher than that of HEMA group (P<0.05). The cell mobility of HEMA group was significantly decreased compared with that of control group (P<0.05), and the cell mobility of HEMA+TPPU group was significantly increased compared with that of HEMA group (P<0.05). After the cells were treated with 2 mmol/L HEMA in the process of odontogenic differentiation, alkaline phosphatase activity was decreased when compared with control group (P<0.05), and the expression of odontogenic differentiation related genes was significantly decreased (P<0.05). The activity of alkaline phosphatase in the HEMA+TPPU group was significantly higher than that in HEMA group (P<0.05), and the expressions of Runx2, DMP-1, and DSPP were also significantly increased (P<0.05). In addition, alizarin red staining showed that the relative calcium contents of HEMA+TPPU group and control group were significantly higher than that of HEMA group (P<0.05). Conclusion: HEMA can inhibit the cell migration and odontogenic differentiation of hDPSCs to some extent, and TPPU can partially reverse the inhibitory effect of HEMA on the cell migration and odontogenic differentiation of hDPSCs.

Key words: soluble epoxide hydrolase inhibitor, HEMA, dental pulp stem cells, cell migration, dontogenic differentiation

LUO Jiaxin, HUANG Jing, WANG Yuling, FENG Yan, DANG Haixia. Effect of Soluble Epoxide Hydrolase Inhibitor on Migration and Odontogenic Differentiation of Human Dental Pulp Stem Cells Exposed to HEMA[J]. Journal of Oral Science Research, 2024, 40(2): 166-171.

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