Diversity of Gingival Crevicular Fluid Flora in Patients with Chronic Periodontitis and Its Correlation with Inflammatory Factors

doi:10.13701/j.cnki.kqyxyj.2025.09.009

Abstract

Abstract: Objective: To explore the diversity of gingival crevicular fluid flora in patients with chronic periodontitis and its correlation with inflammatory factors. Methods: Forty patients with chronic periodontitis admitted to our hospital from January 2024 to October 2024 were divided into mild group (stage Ⅰ and Ⅱ) and severe group (stage Ⅲ and Ⅳ) according to the disease stages, with 20 patients in each group, and 20 healthy subjects were selected as healthy control group. 16S rDNA high-throughput sequencing technique was used to analyze the flora diversity of gingival crevicular fluid and saliva, and the level of serum inflammatory factors was determined to analyze the correlation between them and the flora diversity. Results: There were 1450 OTUs in mild and severe gingival crevicular fluid samples, with 379 and 381 unique OTUs, respectively. There are 1403 OTUs in saliva samples, of which 365 and 379 were unique. There was a significant difference in the relative abundance of multiple flora in gingival crevicular fluid and saliva samples between mild and severe groups (P<0.05), but there was no significant difference in Chao1 index and Shannon index between the two groups (P>0.05). The analysis of the main components of the flora showed that the difference in the Bray-Curtis distance between the mild group and the severe group was statistically significant (P<0.05). The relative abundance of concealed Eubacterium and Corynebacterium in gingival crevicular fluid and saliva samples of patients with chronic periodontitis was positively correlated with IL-6 and IL-1, respectively (P<0.05), and the correlation coefficient of gingival crevicular fluid samples was higher than that of saliva. Conclusion: The severity of chronic periodontitis is related to the change of community structure, but not to the change of species richness and evenness. Moreover, the relative abundance of Bacteroides occulta and Corynebacterium martensii in gingival crevicular fluid of patients with chronic periodontitis is closely related to the level of serum inflammatory factors.

Key words: chronic periodontitis, gingival crevicular fluid, diversity of flora, inflammatory factor

LI Yao, LIU Shuai, LIU Zhifang. Diversity of Gingival Crevicular Fluid Flora in Patients with Chronic Periodontitis and Its Correlation with Inflammatory Factors[J]. Journal of Oral Science Research, 2025, 41(9): 788-794.

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