Abstract: Objective: To prepare and evaluate the Poly (lactide-co-glycolide)/Hydroxylapatite (PLGA/HA) composite scaffold with different proportions. Methods: PLGA/HA composite scaffolds with different HA contents (10%, 20% and 30%) were fabricated by solvent casting/particulate leaching technology, while PLGA without HA was used as control group. The tensile strength and microstructure were detected by using electro-mechanical universal testing machines and scanning electron microscopy (SEM). Human dental pulp stem cells were isolated, cultured and identified. The cells were seeded in the PLGA/HA scaffolds with different proportions. The adhesion properties were measured by DAPI staining. Results: The tensile strength of 10% HA was higher than that of 20% HA, and both of them showed a higher tensile strength than the control group. However, that of 30% HA was lower than control group (P<0.05). Scaffolds with different proportions of PLGA/HA showed high porosity and pore connectivity and the pore size was 100-250 μm investigated by SEM. Cell attachment and number in PLGA/HA after the incubation of 2h, 6h and 12h were significantly higher than the control group (P<0.05), and the cell adhesion ability in 30% HA group was the highest (P<0.05). Conclusions: The present study shows that PLGA/HA composite scaffolds with HA contents may be suitable for tissue engineering. In addition, 10% HA shows a higher tensile strength, while the adhesion ability of hDPSCs in 30% HA is better.