Abstract: Objective: To evaluate the antibacterial properties of experimental nano silver-containing cements (NSC) in the rat caries disease model. Methods: Rat caries disease model was used to simulate the human oral environment. The sample teeth were bonded bracket base with adhesive onto the surface of the buccal half crowns of the first upper premolars. Then the bracket base was fixed at the lingual surface of the front teeth root in rats for a long-time preservation. After one and three months of implantation respectively, 25 samples were taken out from the mouth of the rats under local anesthesia. Then the scanning electron microscope observation was conducted. Results: The caries disease model was successfully built in rats. Through long-term observation, we found that the material samples did not affect the normal eating of rats, and there was almost no materials loss. The scanning electron microscope observation showed that the growth of streptococcus mutans was very active. One month later, Streptococcus mutans scattered or a small amount of them gathered into a cluster on GC Fuji ORTHO LC surface. While three months later, the amount of streptococcus mutans significantly increased and arranged in chains on the material surface. In the groups of NSC2, NSC3 and NSC4, the number of streptococcus mutans presented the downward trend and tended to disperse individually with the increase of nano silver content. Conclusion: The incorporation of silver nanoparticles enhanced the effect of GC Fuji ORTHO LC on the adhesion inhibition and killing of streptococcus mutans.