Effects of Dicalcium Silicate on Murine RAW 264.7 Macrophage Cell Line.

doi:10.13701/j.cnki.kqyxyj.2017.01.007

Abstract

Abstract: Objective: To investigate the proinflammatory response of dicalcium silicate on murine RAW 264.7 macrophage cell line. Methods: qRT-PCR method was used to determine the effects of dicalcium silicate and tricalcium phosphate particles on expressions of TLR2 and TLR9 co-cultured with RAW264.7 for 6 h and 24 h. Results: Scanning electron microscope revealed that RAW264.7cells phagocytosed both dicalcium silicate and tricalcium phosphate particles. Compared with tricalcium phosphate particle group, TLR2 and TLR9 showed no obvious change in dicalcium silicate particles group when co-cultured with RAW264.7 for 6 h. For 24 h, the expression of TLR2 in dicalcium silicate particle group was obviously higher (P<0.001), and the expression of TLR2 was also increased in 10 mg/L tricalcium phosphate group (0.05<P<0.1). Conclusion: TLR2 may be involved in the proinflammatory response of dicalcium silicate murine RAW 264.7 macrophage cell line.

Key words: Dicalcium, silicate, Tricalcium, phosphate, Macrophages, Proinflammatory, response

CLC Number:

R783.1

LAI Shi-xiang, CHEN Liang-jiao, CAO Wei, ZHANG Qing-bin, CUI Shi-man, LI Xing-yang. Effects of Dicalcium Silicate on Murine RAW 264.7 Macrophage Cell Line.[J]. Journal of Oral Science Research, 2017, 33(1): 29-32.

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