Abstract: Objective: To discuss the effects of resveratrol on matrix metalloproteinase-2 (mmp-2) activity and demineralization resistance and to provide experimental evidence for the clinical application of resveratrol. Methods: The concentration gradients of resveratrol were 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L, and 400 μmol/L in the experimental group. 0.2% chlorhexidine was selected as the positive control group and deionized water as the negative control group. 10 μL of each group was mixed with 30 μL extracted odontogenic mmp-2 and incubated for 30 min. Metalloproteinase-2 activity was quantitatively detected with body fluid matrix metalloproteinase-2 activity assay after 20 min, and each group was repeated five times. After going through the pH cycle, electron microscopy and laser scanning confocal 3D image were used to observe dentin demineralization depth. Results: Resveratrol with the concentration above 50 μmol/L showed significant inhibitory effect. And metalloproteinase-2 activity inhibition rate in groups with the concentration of 100 μmol/L, 200 μmol/L, and 400 μmol/L showed no significant difference compared to the positive group. Confocal electron microscopy 3D image displayed that Res dyeing depth in the 100 μmol/L group was significantly lower than that in the negative control group. And statistical analysis showed the same results (P<0.05). But there was no significant difference between 100 μmol/L group and the positive control group. Conclusion: Resveratrol can inhibit the activity of matrix metalloproteinase-2 and dentin demineralization.

Key words: Resveratrol, Matrix, metalloproteinases, Demineralized, dentin