Effects of Chronic Sleep Deprivation on Expression of MMP-2 and MMP-9 in Condyle of Rats

doi:10.13701/j.cnki.kqyxyj.2021.11.011

Abstract

Abstract: Objective: To investigate the effects of chronic sleep deprivation and sleep recovery on the development of temporomandibular joint degeneration in rats and the changes in the expression of MMP-2 and MMP-9. Methods: MMPM method was used to establish the rat model of chronic sleep deprivation for 1, 2, 3, and 4 weeks and the open field test was used to verify. The HE staining was used to observe the changes of rat’s condylar cartilage tissue and immunohistochemical method was used to detect the expression of MMP-2 and MMP-9 in condylar cartilage. Results: MMPM could successfully induce the rats into the chronic sleep deprivation state. HE staining showed that the experimental group’s condyle cartilage had degenerative changes. Immunohistochemical results showed that the positive expression intensity of MMP-2 and MMP-9 in the experimental group at each time point was higher than that in the control group (P<0.05), and the positive expression intensity in the recovery group at each time point was lower than that in the experimental group (P<0.05). Conclusion: Chronic sleep deprivation can cause degeneration changes of condyle cartilage in rats, and the secretion of MMP-2 and MMP-9 by chondrocytes is involved in the degeneration process.

Key words: chronic sleep deprivation, temporomandibular joint, condylar cartilage, matrix metalloproteinase-2, matrix metalloproteinase-9

LIU Yang, BAI Yuehui, CUI Yulan, ZHAO Chen. Effects of Chronic Sleep Deprivation on Expression of MMP-2 and MMP-9 in Condyle of Rats[J]. Journal of Oral Science Research, 2021, 37(11): 1009-1016.

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