Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions.

doi:10.13701/j.cnki.kqyxyj.2017.06.018

Abstract

Abstract: Objective: To compare the relative expression of miR-146a-5p in chronic periapical lesions and healthy periodontal ligaments, and discuss the role and clinical significance of miR-146a-5p in the etiology of chronic apical periodontitis. Methods: Sixteen periapical tissues from chronic apical periodontitis and 8 normal periodontal ligament tissues from extracted healthy teeth for orthodontic reasons were selected. The degree of inflammatory infiltration was detected by Hematoxylin- Eosin staining. The relative expression of miR-146a-5p was detected by real-time PCR. All the data were analyzed by SPSS 18.0 software package. Results: According to the result of HE staining, there was no inflammation in control group, but large numbers of inflammatory cells were observed in periapical lesions. MiR-146a-5p was detected by real-time PCR in two groups, and the level of miR-146a-5p relative expression in periapical lesions was significantly higher than that of the healthy periodontal ligaments (P＜0.05). Conclusion: MiR-146a-5p might closely relate to the occurrence and development of chronic apical periodontitis.

Key words: Chronic apical periodontitis , miR-146a , Hematoxylin-Eosin staining , RT-PCR

CLC Number:

R781.3

SHAO Li-na, WANG Si-mo, LI Xiao-lin, QIU Li-hong, YU Bo.. Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions.[J]. Journal of Oral Science Research, 2017, 33(6): 650-653.

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