Effect of RGD/biomimetic Calcium Phosphate Coatings on Adhesion, Proliferation and Differentiation of MC3T3-E1 Cells on Titanium.

doi:10.13701/j.cnki.kqyxyj.2017.07.002

Abstract

Abstract: Objective: To evaluate the effect of biomimetic calcium phosphate coatings containing RGD on adhesion, proliferation and differentiation of MC3T3-E1 cells. Methods: A 2-step approach was adopted to prepare biomimetic calcium phosphate coatings on the surface of titanium. The characterization of the coatings was analyzed by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy, and X-ray diffractionometry. The RGD was incorporated into coatings through co-precipitation. MC3T3-E1 cells were seeded on Ti, Ti/ biomimetic calcium phosphate coatings, and Ti/RGD/biomimetic calcium phosphate coatings, respectively. The morphology of MC3T3-E1 cells cultured on samples was observed by SEM. The attachment of MC3T3-E1 cells on various samples was determined by crystal violet staining assay. The proliferation of MC3T3-E1 cells was determined by MTT after cultured for 1, 3 and 7 days. The ALP activity of the MC3T3-E1 cells was measured after cells cultured on various samples for 3, 7 and 14 days. Results: The results of SEM and crystal violet assay showed that the adhesion of cells growing on RGD/biomimetic calcium phosphate coatings was greater than that of control groups. Moreover, MTT test showed that the proliferation of cells growing on RGD/biomimetic calcium phosphate coatings was greater compared with that in control groups. In addition, the ALP activity of MC3T3-E1 cells cultured on RGD/biomimetic calcium phosphate coatings was higher than that in control groups. Conclusion: RGD/biomimetic calcium phosphate coatings can promote adhesion, proliferation, and differentiation of MC3T3-E1 cells.

Key words: RGD , Biomimetic calcium phosphate coatings, MC3T3-E1 cells , Adhesion, Proliferation , Differentiation

CLC Number:

R782.12

CAO Ya-zhou, CHENG Wei, HU Qin-gang.. Effect of RGD/biomimetic Calcium Phosphate Coatings on Adhesion, Proliferation and Differentiation of MC3T3-E1 Cells on Titanium.[J]. Journal of Oral Science Research, 2017, 33(7): 693-697.

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