Cytocompatibility of Ultrasonic Micro-arc Oxidation-Dopamine-Cyclo (RGD) Coating on Pure Titanium.

doi:10.13701/j.cnki.kqyxyj.2017.07.004

Abstract

Abstract: Objective: To discuss the effect of Cyclo (RGD) coating on the surface of pure titanium implant on the biological behavior of osteoblasts. Methods: The specimens were divided into 3 groups: pure titanium ultrasonic micro-arc-oxidation (group M); pure titanium ultrasonic micro-arc-oxidation-dopamine (group P); and pure titanium ultrasonic micro-arc-oxidation-dopamine-Cyclo (RGD) (group R). Scanning electron microscope was used to observe the surface morphology. The surface elements of three groups were analyzed by energy spectrometer. CCK-8 experiment was used to detect the adhesion rate and proliferation of osteoblasts in different time points. The spread of osteoblasts on the surface of three groups was observed by laser confocal microscopy. Results: The observation of scanning electron microscope and the spectrum of coating displayed successful loading. And the results of CCK-8 showed that the OD value of group R was higher than those of group M and group P on tested time points (P<0.05). Confocal laser microscope showed the cytoskeleton form. During the same period, the spread area and the number of microfilament and pseudopodia of both group P and group R were better than those of group M. And the group R was the most conducive to cell stretch. Conclusion: The ultrasonic micro-arc oxidation-dopamine-cyclo (RGD) coating showed a good effect on early adhesion and proliferation of osteoblasts.

Key words: Pure titanium , Micro-arc oxidation, Dopamine , Cyclo (RGD)

CLC Number:

R783.1

ZHOU Tian-yuan, LI De-chao, Li Mu-qin, Li Shan-chang, Liu Shuang, Ma Jing, Tian Lei, Yu Jia-ying, Wen Xin.. Cytocompatibility of Ultrasonic Micro-arc Oxidation-Dopamine-Cyclo (RGD) Coating on Pure Titanium.[J]. Journal of Oral Science Research, 2017, 33(7): 703-706.

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