Expression of Angiogenic-associated and Lubricating-associated Factors in Synovial Fibroblasts of Temporomandibular Joint Osteoarthritis

doi:10.13701/j.cnki.kqyxyj.2018.03.014

Abstract

Abstract: Objective: To investigate the expression of angiogenic-associated factors and lubricating-associated genes in the synovial fibroblasts of osteoarthritis (OA). Methods: Synovial tissues were collected from the temporomandibular joint of OA patients, and synovial fibroblasts were separated and cultured by tissue culture method. Flow cytometric analysis was used to examine the expression of CD34 and CD44. The protein expressions of VEGF and TSP1 were detected by western blotting. Real-time PCR was used to examined the gene expression of PRG4,HAS2 and HAS3. Results: The expressions of VEGF,CD34 and HAS3 were significantly increased in the synovial cells of OA, but TSP1, PRG4 and HAS2 expression were decreased. Conclusion: Proangiogenic factors were increased, and the lubricating-associated factors were decreased in the synovial cells of temporomandibular joint OA, suggesting that the synovium might play a beneficial role in the pathogenesis of OA by regulating the expression of these factors.

Key words: Lubricin, Hyaluronan synthase, Osteoarthritis, Angiogenesis

GUO Hui-lin, FANG Wei, DENG Mo-hong, LONG Xingsup>1,2*. Expression of Angiogenic-associated and Lubricating-associated Factors in Synovial Fibroblasts of Temporomandibular Joint Osteoarthritis[J]. Journal of Oral Science Research, 2018, 34(3): 270-273.

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