Comparison of Expression of Mesenchymal Stem Cell Surface Markers on Synovial Mesenchymal Cells of Patients with Temporomandibular Joint Disease

doi:10.13701/j.cnki.kqyxyj.2018.11.024

Abstract

Abstract: Objective: To detect whether mesenchymal stem cells (MSCs) markers (CD34, CD73, CD90, CD105, CD271) were expressed in synovial mesenchymal cells(SMCs) of temporomandibular joint and to compare their expression in SMCs of temporomandibular joint from human osteoarthritisis and condylar hypertrophy. Methods: SMCs of temporomandibular joint were isolated using explants culture method and MSCs were obtained by the repeated adherent culture method. Then, the third generation cells were characterized and the expression of CD73, CD90, CD105, CD271, and CD34 were qualified by flow cytometry. Results: Fluorescence-activated cell sorting (FACS) analysis showed more than 90% MSCs from human iliac bone and SMCs from human temporomandibular joint expressed CD73, CD90, and CD105, less than 1% cells expressed CD271, and the quantification of CD34 was 0.47%-30%. Conclusion: MSCs from human iliac bone and SMCs of temporomandibular joint from human were positive for CD73, CD90, and CD105, while the expression of CD34 and CD271 were low. SMCs of temporomandibular joint with osteoarthritisis contained more cells positive for CD34 antigens than those from temporomandibular joint condylar hypertrophy.

Key words: Bone marrow mesenchymal stem cells, Synovial mesenchymal cells of temporomandibular joint, Mesenchymal stem cell surface markers, Osteoarthritisis of temporomandibular joint

LU Hong-yan, LI Jian, LONG Xing. Comparison of Expression of Mesenchymal Stem Cell Surface Markers on Synovial Mesenchymal Cells of Patients with Temporomandibular Joint Disease[J]. Journal of Oral Science Research, 2018, 34(11): 1249-1252.

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