Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway

doi:10.13701/j.cnki.kqyxyj.2016.09.005

Abstract

Abstract: Objective: To reveal the mechanism of GBE promoting osteogenic differentiation of rat BMSCs. Methods: 150mg/L GBE was applied to the third generation of rat BMSCs, while the inhibitor group underwent a pretreatment of SB203580, which was the specific inhibitor of P38 MAPK signaling pathway. The results were evaluated by ARS dye, ALP activity assay and RT-PCR. Results: Statistically significant difference was found between the control group and the inhibitor group. In the inhibitor group, extracellular calcium deposition, ALP activity and the expression of osteogenic-related genes including ALP, BMP-2, OCN and Runx2 were lower than the control group. Conclusion: GBE promoted osteogenic differentiation of rat BMSCs by upregulating the P38 MAPK signaling pathway.

Key words: Ginkgo biloba extract, Bone marrow mesenchymal stem cells, P38 MAPK signaling pathway, Osteogenic differentiation

CLC Number:

X703.5

SHI Shu-man , YUAN Hao-tian , ZHANG Xiao-xiao , WU Zhe. Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway[J]. Journal of Oral Science Research, 2016, 32(9): 912-915.

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