Establishment and Evaluation of A Rabbit Model of Oral Keratinized Soft Tissue Wound.

doi:10.13701/j.cnki.kqyxyj.2018.05.025

Abstract

Abstract: Objective: To establish an animal model of oral keratinized soft tissue wound with scientificalness, repeatability, comparability, reliability, and standardization, and to evaluate its morphology and histology. Methods: 3-month-old male Japanese rabbits were divided randomly into 4 groups: 3d, 7d, 14d and 21d groups (n=3). Keratinized soft tissue wound was generated by using a punch-biopsy instrument (diameter 10mm) at the middle third of hard palate, respectively far from bilateral mucosal margin 2.5mm, and away from maxillary teeth 10mm. Three soft tissues were randomly chosen and cut as control group. Gross observation and wound healing analysis were performed after 3, 7, 14, and 21 days. HE staining and VG staining were used to observe inflammatory reaction, healing of the epithelial thickness, and collagen formation, after collected the specimen on the surgery area. Results: Gross observation: the inflammatory reaction after 3 days was the most severe. As time went by, the wound was gradually healed. Histological observation: the number of inflammatory cells was the most after 3 days. Keratinized epithelium and fibrous connective tissue were gradually healed after 7 days, with cell proliferation and formation of granulation tissue. The wound thickness in each group had no statistical difference. The wound healing rate: 3d< 7d<14d<21d (P<0.01). The grades of inflammatory reaction of both 3d and 7d groups were higher than those of control group, 14d, and 21d groups (P<0.05). The average epithelial thickness of 14d group was narrower than those of control group and 21d group (P<0.05). The grades of the density of collagen fibres of both 3d and 7d groups were lower than that of control group (P<0.05), and that of 21d group was higher than that of control group (P<0.05). Conclusion: An animal model of oral keratinized soft tissue wound was successfully established, which laid a foundation for researching the way, the material, and the mechanism of promoting healing of oral keratinized soft tissue in the future.

Key words: Wound healing, Animal model, Rabbits, Hard palate, Connective tissue

WANG Lei, FANG Ya-wei. Establishment and Evaluation of A Rabbit Model of Oral Keratinized Soft Tissue Wound.[J]. Journal of Oral Science Research, 2018, 34(5): 567-571.

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