Abstract: Objective: To evaluate the practicability of autogenous fresh demineralized tooth graft (Auto-FDT) in preservation of extraction sites and periodontal regeneration. Methods: The upper and lower third incisors of two beagle dogs were extracted, and then the eight extraction sockets were randomly divided into two groups: control group (4 sites), natural healing after tooth extraction, no material implantation; experimental group (4 sites), the Auto-FDT was put into extraction sockets after tooth extraction with barrier membrane covered. Meanwhile, ten furcation bone defects were established at the mandibular premolars. And the defects were randomly divided into two groups: the control group (4 sites), natural healing withought bone graft; the experimental group, implanted the Auto-FDT and membrane. After 12 weeks, the animals were euthanized and the jaw specimens were taken. The effect of bone regeneration was evaluated through CBCT and histological analysis. Results: CBCT images showed that the average gray value of the experimental group of extraction sites was significantly higher than that of the control group, and the average gray value of the experimental group of furcation lesions was significantly higher than that of the control group. Pathological sections showed that grafting of the Auto-FDT maintained the regeneration space well, and active new bone formation was observed around the granules. Part of the Auto-FDT particles were replaced. Conclusion: Autogenous fresh demineralized tooth graft is a promising bone substitute material for periodontal regeneration.

Key words: autogenous fresh demineralized tooth graft, autograft, periodontal regeneration, site preservation, furcation lesion, bone defect