Effect of bFGF-loaded Thermosensitive Hydrogel on Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in Vitro

doi:10.13701/j.cnki.kqyxyj.2021.12.017

Abstract

Abstract: Objective: To synthesize bFGF-loaded chitosan/β-glycerophosphate/gelatin thermosensitive hydrogel and study its effect on osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Methods: The chitosan/β-glycerophosphate/gelatin thermosensitive hydrogel was prepared and characterized by electron microscope after freeze-drying. The toxicity of hydrogel was detected. The CCK-8 method was used to screen out the optimum concentration of thermosensitive hydrogel loaded with bFGF to promote BMMSCs proliferation. The effects of bFGF-loaded thermosensitive hydrogel on osteogenic differentiation of BMMSCs were confirmed by ALP staining, alizarin red staining, and semi-quantitative analysis of the calcium nodules. Real-time fluorescence quantitative PCR was used for detecting the effect of bFGF-loaded thermosensitive hydrogel on osteogenesis-related gene expression of BMMSCs. Results: The chitosan/β-glycerophosphate/gelatin thermosensitive hydrogel was successfully prepared, and the pore size of the freeze-drying hydrogel under scanning electron microscope was between 20 and 80 μm. The results of live/dead cells staining showed that the chitosan/β-glycerophosphate/gelatin thermosensitive hydrogel had good biocompatibility. The bFGF-loaded thermosensitive hydrogel could significantly promote the proliferation of BMMSCs in a dose-dependent manner, and the optimum concentration was 100 ng/mL. In the bFGF-loaded thermosensitive hydrogel group, ALP expression and calcium nodule formation (P<0.05)both increased. Also, the expression of osteogenesis-related genes ALP, BMP-2, and Runx2 increased in the bFGF-loaded thermosensitive hydrogel group. Conclusion: The bFGF-loaded thermosensitive hydrogel can promote the osteogenic differentiation of rat BMMSCs.

Key words: bone defect, hydrogel, bFGF, bone marrow mesenchymal stem cells

SI Chao, ZOU Xinying, LIU Yue, ZHANG Peipei, XU Yishan, HAO Yifan, GONG Baijuan. Effect of bFGF-loaded Thermosensitive Hydrogel on Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in Vitro[J]. Journal of Oral Science Research, 2021, 37(12): 1139-1144.

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