Role of ALPL in Regulating Tube Formation of Endothelia Cells by Exosomes from BMMSCs

doi:10.13701/j.cnki.kqyxyj.2021.05.017

Abstract

Abstract: Objective: To explore the effect of ALPL in BMMSCs on endothelia cells (ECs) tube formation. Methods: Multilineage-differentiation was performed on normal human BMMSCs (Nor-hBMMSCs) and HPP patient BMMSCs (HPP-hBMMSCs). Then Nor-hBMMSCs, HPP-hBMMSCs, and ALPL overexpressed HPP-hBMMSCs (HPP+Plenti-ALPL) were cocultured with ECs. The vascular capacity of each groups was measured by tube formation experiment. To block exosomes secretion, HPP+Plenti-ALPL group was administrated with GW4869 before the tube formation ability was measured. To evaluate the tube formation capacity, ECs were treated with exosomes derived from Nor-hBMMSCs, HPP-hBMMSCs, and HPP-hBMMSCs+Plenti-ALPL, respectively. Results: The ALPL deficiency significantly impaired hBMMSCs induced tube formation of ECs. While the angiogenesis capacity of ECs in HPP-hBMMSCs was rescued by overexpression of ALPL, and such function could be blocked by inhibited exosomes secretion. Conclusion: ALPL plays a significant regulatory role in BMMSCs induced tube formation of vascular endothelial cell in an exosomes-dependent manner

Key words: bone marrow mesenchymal stem cells, exosomes, alkaline phosphatase, tube information

DONG Jiayi, ZHAO Jiangdong, ZHAO Wanmin, LIU Wenjia, LI Dehua. Role of ALPL in Regulating Tube Formation of Endothelia Cells by Exosomes from BMMSCs[J]. Journal of Oral Science Research, 2021, 37(5): 462-467.

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