Chir99021 Regulates Osteogenic/Dentinogenic Differentiation of Dental Pulp Stem Cells through Wnt/β-catenin Signaling Pathway

doi:10.13701/j.cnki.kqyxyj.2021.12.019

Abstract

Abstract: Objective: To investigate the effect of osteogenic and dentiparous differentiation of human dental pulp stem cells through Wnt/β-catenin signaling pathway controlled by Chir99021 and its possible mechanism. Methods: HDPSCs were purified and cultured. DSP and Vimentin were detected by immunofluorescence. The effects of different concentrations of Chir99021 on HDPSCs proliferation was detected by CCK-8. Three groups were prepared according to the culture medium, as follows: blank control group was normal culture, experimental control group was osteogenic induction culture, and experimental group was osteogenic induction and Chir99021 culture. Lycopene staining method was used to detect calcium deposits. qPCR was used to detect Axin2, β-catenin, and GSK-3β in day 5, 10, and 15. Western blotting was used to detect Runx2, ALP, OC, DSPP and collagen Ⅰ in day 5, 10, and 15. Results: DSP and Vimentin were positive in purified HDPSCs. The best concentration of Chir99021 was 2000 nmol/L. The calcium deposits of experimental group were significantly more than other groups. β-catenin and Axin2 of experimental group was significantly higher than those of other groups (P<0.05). GSK-3β of experimental group was significantly lower (P<0.05). Runx2 and ALP were significantly expressed in the control group and experimental group (P<0.05). OC of experimental group was significantly higher (P<0.05). DSPP and collagen Ⅰ were significantly expressed in the experimental group (P<0.05). Conclusion: Osteogenic and dentiparous differentiation of HDPSCs were activated by Wnt/β-catenin signaling pathway controlled by Chir99021.

Key words: dental pulp stem cells, Chir99021, Wnt/β-catenin signaling pathway, osteogenic differentiation, dentiparous differentiation

XIE Huilan, FANG Fang, LIN Yi. Chir99021 Regulates Osteogenic/Dentinogenic Differentiation of Dental Pulp Stem Cells through Wnt/β-catenin Signaling Pathway[J]. Journal of Oral Science Research, 2021, 37(12): 1150-1155.

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