Study on Cytocompatibility, Osteogenic Potential, and Antibacterial Activity of Three Root Canal Sealers in Vitro

doi:10.13701/j.cnki.kqyxyj.2022.11.014

Abstract

Abstract: Objective: To evaluate the in vitro cytocompatibility and osteogenic and antibacterial activity of iMTA, BioRoot RCS, and AH Plus. Methods: The control group and experimental group of three root canal sealers were set. The extraction was gradient diluted by 100%, 75%, 50%, and 25%, respectively. L929 cells were cultured with different concentrations of extracts. The A values at 24, 48, and 72h were determined by CCK-8, and the relative proliferation rate of each group was calculated. MC3T3-E1 cells were cultured with extracts. The alkaline phosphatase (ALP) activity and mineralization-promoting nodule formation potential were compared after 7 days and 28 days. Using direct contact method, fresh sealer was mixed with Enterococcus faecalis solution to compare the antibacterial activity of each group. Results: With the increase of extraction concentration, the relative proliferation rate of cells were decreased. The relative proliferation rates of iMTA and BioRoot RCS group were higher than that of AH plus group under the same contact time and concentration (P<0.01). The ALP activity and mineralization-promoting nodule formation ability of iMTA and BioRoot RCS were significantly higher than those of AH plus (P<0.01), and there was no significant difference between iMTA and BioRoot RCS groups (P<0.01). Compared with the control group, iMTA significantly reduced the number of Enterococcus faecalis (P<0.01). Conclusion: The cytotoxicity of iMTA is negatively correlated with the concentration of the extract and positively correlated with the contact time. iMTA and BioRoot RCS have similar osteogenic energy, and have certain antibacterial effect on Enterococcus faecalis.

Key words: root canal sealer, cytocompatibility, osteogenesis, antibacterial

YANG Xiliang, ZHENG Tianxia, YANG Nuoya, YIN Zihan, BAI Yuhong. Study on Cytocompatibility, Osteogenic Potential, and Antibacterial Activity of Three Root Canal Sealers in Vitro[J]. Journal of Oral Science Research, 2022, 38(11): 1071-1075.

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