Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro

doi:10.13701/j.cnki.kqyxyj.2022.01.010

Abstract

Abstract: Objective: To study the effect of chlorhexidine-loaded gellan gum hydrogel against Enterococcus faecalis and biofilm in vitro. Methods: The gellan gum containing hydroxyapatite was prepared by adding crosslinking agent (calcium ion), and chlorhexidine was effectively loaded. The effect of hydrogels against Enterococcus faecalis in vitro was evaluated by measuring bacterial growth curves and scanning electron microscopy, and their inhibiting the bacterial biofilms was observed by quantitative crystal violet staining and confocal laser scanning microscope. Results: As the concentration of chlorhexidine in the hydrogel increased, the antibacterial activity gradually increased. When the concentration of chlorhexidine was 50 μg/mL, it could significantly inhibit bacterial growth and biofilm formation. When the concentration was 250 μg/mL, it could show significant bactericidal effect. Conclusion: The gellan gum hydrogel loaded with chlorhexidine presented great effects on inhibiting the growth of Enterococcus faecalis and removing their biofilm. It might be a novel injectable biomaterial to disinfect root canals and promote the healing of refractory periapical periodontitis.

Key words: gellan gum, hydrogel, Enterococcus faecalis, antibacterial, biofilm

XU Laijun, YUAN He, LI Jiyao. Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro[J]. Journal of Oral Science Research, 2022, 38(1): 45-50.

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