β-Lapachone Suppresses OSCC Proliferation and Induces Cell Apoptosis through ROS Generation

doi:10.13701/j.cnki.kqyxyj.2022.01.009

Abstract

Abstract: Objective: To evaluate the expression level of NQO1 in OSCC, and investigate the effect of β-Lapachone on proliferation, apoptosis, and ROS generation in OSCC. Methods: The expression of NQO1 was detected by IHC staining in 50 cases of OSCC tissues and 20 cases of normal oral tissues. Cell viability was detected using CCK-8 and colony formation and Edu incorporation assay after β-Lapachone treatment. The effect of β-Lapachone on cell apoptosis was detected by flowcytometry. ROS generation was determined by DCFH-DA method. ROS inhibitor (NAC) was applied to determine the effect of ROS generation on OSCC cell proliferation. Results: IHC staining showed that the positive and strongly positive rate of NQO1 was significantly higher than that of normal tissues (P<0.05). The expression of NQO1 was correlated with tumor size and histological grade. The results of CCK-8 and colony formation and Edu incorporation assay showed that β-Lapachone suppressed OSCC cell proliferation. In addition, Annexin V/7-AAD staining showed that β-Lapachone induced OSCC cell apoptosis. Additionally, β-Lapachone could induce ROS generation. ROS inhibitor (NAC) could partially attenuate the effect of β-Lapachone on cell proliferation. Conclusion: The expression of NQO1 was correlated with OSCC development and progression. β-Lapachone regulated cell proliferation and apoptosis through regulating ROS generation.

Key words: OSCC, NQO1, β-Lapachone, ROS, proliferation, apoptosis

LIN Jing, JIN Chengri, XUAN Yunze. β-Lapachone Suppresses OSCC Proliferation and Induces Cell Apoptosis through ROS Generation[J]. Journal of Oral Science Research, 2022, 38(1): 39-44.

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