Expression of High-mobility Group Box B1 in Human Dental Pulp Cells during OdontoblasticDifferentiation.

Abstract

Abstract: Objective: To investigate the expression of high-mobility group box B1 (HMGB1) in human dental pulp cells (hDPCs) during odontoblastic differentiation and explore the role of HMGB1 in dental repair. Methods: hDPCswere isolated and cultured in odontoblastic induction medium for 0, 3, 7, 11 and 14 days respectively. Important mineralization-related genes such asdental matrix protein-1 (DMP-1), dental sialophosphoprotein (DSPP) and alkaline phosphatase (ALP) were tested by real-time polymerase chain reaction. Thelevels of mRNA and protein of HMGB1 and the ALP activity were also detected. The expression pattern of HMGB1 during the hDPCsodontoblastic differentiation was detected by immunoflurescence staining. Results: The mRNA levels of DMP1 and DSPP and ALP activity were up-regulated during the hDPCsodontoblastic differentiation on days 7, 11 and 14 (P<0.05). The mRNA level of HMGB1 was significantly increased on days 11 and 14 (P<0.05). The intracellular protein level of HMGB1 was down-regulated and HMGB1 was translocated from nucleus to the cytoplasm during the procedure of odontoblastic differentiation. Conclusion: HMGB1, DMP1, DSPP/DSP and ALP showed analogously expression trend in mRNA level during the hDPCsodontoblastic differentiation. HMGB1 may play an important role in the odontogenic differentiation of hDPCs and dental repair.

CLC Number:

R780.1

CUI Chun, QI Sheng-cai.. Expression of High-mobility Group Box B1 in Human Dental Pulp Cells during OdontoblasticDifferentiation.[J]. Journal of Oral Science Research, 2015, 31(11): 1100-1103.

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