Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113.

Abstract

Abstract: Objective: To investigate the effect of dracorhodin perchlorate (DP) on the cell proliferation and apoptosis and to discuss relevant signaling pathways in human tongue squamous carcinoma cell line Tca-8113 . Methods: The Tca8113 cells were used as research object in this experiment. The growth, apoptosis and the expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase 1(HO-1) were detected in human tongue carcinoma cell line(Tca-8113), using cell counting kit-8 (CCK-8), the flow cytometry and Western blot to assay. All statistical analysis was performed using the SPSS 17.0 statistical package. Results: CCK-8 assay proved that there was a much pronounced decrease in cell viability on treatment with DP compared with control group and the time- and dose-dependent effects of treatment with DP on the growth of cells (P<0.05). Flow cytometric analysis and Western blot demonstrated that DP promoted cell apoptosis (P<0.05) and elevated the expression of Nrf2 and HO-1 proteins in Tca-8113 cells. Conclusion: DP inhibited tongue squamous carcinoma Tca-8113 cells growth and induced apoptosis, which may be relevant with the activation of Nrf2/HO-1signaling pathway in oral cancer cells. DP could be developed as an agent against OSCC.

Key words: Dracorhodin perchlorate , Nrf2/HO-1 , Tca-8113, Apoptosis

CLC Number:

R780.1

ZHOU Cai-ran, GUO Shu-juan, ZENG Jin, et al.. Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113.[J]. Journal of Oral Science Research, 2015, 31(3): 254-257.

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