Expression of Autophagy-related Gene LC3Av1 in Oral Squamous Cell Carcinoma and the Effection of Methylation.

Abstract

Abstract: Objective: To detect the expression of autophagy-related gene LC3Av1 in OSCC and the methylation status of LC3Av1, to preliminary discuss the relationship between autophagy and tumorigenesis of OSCC. Methods: The total RNA of 18 OSCC cell lines were extracted and detected LC3Av1 expression at the transcriptional level. Then the methylation status of LC3Av1 transcriptional start site was detected by COBRA. Results: Only 3 cell lines expressed 16.7% in 18 cell lines of OSCC (expressive rate was 16.7%),while LC3B expressed in all lines .And LC3Av1 methylation was detected in 17 cell lines. Conclusion: The expression of LC3Av1 decreased in OSCC suggested that the tumorigenesis of OSCC might relate to obstruction of LC3Av1 dependent autophagy. And the inactivation of LC3Av1 was associated with hypermethylation of LC3Av1 gene.

Key words: Autophagy, OSCC, LC3Av1 , Methylation

CLC Number:

R739.8

WANG Qian, MA Jin-bao, BAI Hua. Expression of Autophagy-related Gene LC3Av1 in Oral Squamous Cell Carcinoma and the Effection of Methylation.[J]. Journal of Oral Science Research, 2015, 31(4): 373-376.

References

[1] Virgin HW, Levine B. Autophagy genes in immunity [J]. Nat Immunol, 2009, 10(5)∶461-470
[2] Kabeya Y, Mizushima N, Yamamoto A, et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation [J]. J Cell Sci, 2004,117(13)∶2805-2812
[3] Wu J, Dang Y, Su W, et al. Molecular cloning and characterization of rat LC3A and LC3B–two novel markers of autophagosome [J]. Biochem Biophys Res Commun, 2006, 339(1)∶437-442
[4] H Bai, J Inoue, T Kawano, et al. A transcriptional variant of the LC3A gene is involved in autophagy and frequently inactivated in human cancers [J]. Oncogene, 2012, 31(40)∶4397-4408
[5] Fleming A, Noda T, Yoshimori T, et al. Chemical modulators of autophagy as biological probes and potential therapeutics [J]. Nat Chem Biol,2011,7(1)∶9-17
[6] Mathew R, Karantza-Wadsworth V, White E. Role of autophagy in cancer [J]. Nat Rev Cancer,2007,7(12)∶961-967
[7] 白宇,全海英,张桐菲,等.顺铂诱导的自噬在口腔鳞癌Tca83细胞中的作用[J].口腔医学研究,2012,28(6)∶566-569
[8] 赵轩一,张泽兵,白宇,等.自噬相关基因Beclinl和MAPlLC3在口腔鳞状细胞癌中的表达及意义[J].口腔医学研究,2012,28(1)∶61-64
[9] 金淑芳,蒋灿华. 细胞自噬相关蛋白8及其连接系统与头颈部恶性肿瘤[J].国际口腔医学杂志,2014,41(2)∶195-198
[10] Mizushima N, Levine B, Cuervo AM, et al. Autophagy fights disease through cellular self-digestion [J]. Nature, 2008,451(7182)∶1069-1075
[11] Virgin HW, Levine B. Autophagy genes in immunity [J]. Nat Immunol, 2009, 10(5)∶461-470
[12] Takamura A, Komatsu M, Hara T, et al. Autophagy-deficient mice develop multiple liver tumors [J]. Genes Dev,2011,25(8)∶795-800
[13] Yang S, Wang X, Contino G, et al. Pancreatic cancers require autophagy for tumor growth[J]. Genes Dev, 2011, 25(7)∶717-729
[14] Esteller M. Epigenetics in cancer [J]. N Engl J Med, 2008, 358(11)∶1148-1159
[15] Schnekenburger M, Grandjenette C, Ghelfi J, et al. Sustained exposure to the DNA demethylating agent, 2’-deoxy-5-azacytidine, leads to apoptotic cell death in chronic myeloid leukemia by promoting differentiation,senescence, and autophagy [J]. Biochem Pharmacol, 2011, 81(3)∶364-378