Abstract: Objective: To prepare the electrospun fibers which contained parathyroid related peptide (rhPTH (1-34)) and to study its slow release on the proliferation and differentiation of osteoblasts. Methods: Electrospinning fibers (PLLA/rhPTH (1-34)) containing slowing released rhPTH (1-34) were prepared. The appearance and the morphology of the fibers were observed with scanning electron microscopy (SEM). The release kinetics was measured by UV spectrophotometry in vitro. The proliferation activity of MC3T3-E1 cells influenced by PLLA/rhPTH (1-34) was determined through Thiazole blue method (MTT). The differentiation of MC3T3-E1 cells was determined by alkaline phosphatase (ALP). Results: The sustained-release efficiency day of the spinning fibers in vitro was 21. PLLA/rhPTH(1-34) sustained-release fiber could obviously promote the proliferation and differentiation of MC3T3-E1 cells (P<0.05). Conclution: PLLA/rhPTH (1-34) loaded fibers can effectively release drugs and promote the proliferation and differentiation of MC3T3-E1 cells.

Key words: Electrostatic spinning, RhPTH (1-34), MC3T3-E1