Mechanism of Osteogenesis of BMSCs under Continuous Mechanical Tension Force

Abstract

Abstract: Objective: To study the role of p38MAPK signal pathway in regulating osteogenic differentiation of C57BL/6J mouse BMSCs under the continuous mechanical tension force. Methods: The BMSCs of primary culture were divided randomly into control group and tension inhibitor group. The tension inhibitor group was pretreated for 1 hour with SB203580 which was one of p38MAPK pathway specific inhibitor, and then loaded with mechanical stimulation by Flexercell. The tension force (0.5Hz, 0.8%) was inflicted to both groups at 0, 30 and 60 min, respectively. p38MAPK and P-p38MAPK protein were detected by Western blot, and the expression of osteogenic genes ALP, COLI, OCN were detected by real time-PCR. Results: C57BL/6J mouse BMSCs grew well with typical shuttle shape and capacities of multi-differentiation. Real time-PCR and Western blot results showed that the expression of ALP, COLI, OCN mRNA and P-p38MAPK protein in the control group was significant different among different loading time points. The mRNA and P-p38MAPK protein expression in control group was markedly higher than those in the tension inhibitor group at different time points (P<0.05). Conclusion: The continuous tension force (0.5Hz, 0.8%) can promote osteogenesis of BMSCs through p38MAPK signal pathway.

Key words: Continuous tension force, Bone marrow mesenchymal stem cells (BMSCs), Osteogenesis, P38MAPK signal pathway

CLC Number:

R329.2

LIU Xiao-ya, XIAO Wen-lin, YU Bao-jun. Mechanism of Osteogenesis of BMSCs under Continuous Mechanical Tension Force[J]. Journal of Oral Science Research, 2015, 31(8): 775-.

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