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18 January 2019, Volume 35 Issue 1 Previous Issue    Next Issue

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New Concept of Tooth Restoration CHEN Zhi, CHEN Rui-tian 2019, 35(1): 1-9.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.001 Abstract ( 418 )   PDF (1091KB) ( 529 )   With the advancement of dental materials and adhesive technology, a great deal of evidence-based medicines show that there is no significant differences for longevity between direct and indirect restorations. The introduction and development of minimal invasive approach of restorative treatment makes more and more dentists realize the importance of retaining vital pulp and healthy tooth tissue. In this article, the minimal invasive principle and restorative cycle of restorative dentistry are described. References | Related Articles | Metrics

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Research Progress on Autogenous Teeth Bone in Dental Implantation ZHANG Yong, XIA Hai-bin 2019, 35(1): 10-12.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.002 Abstract ( 415 )   PDF (768KB) ( 284 )   In the past decades, restorations supported by dental implants have become the priority treatment option for patients with partial or total edentulous. However, bone defect after teeth removal will increase the risk of dental implants. Many bone substitutes have been used to solve bone defect in clinic, but the substitutes exist different shortcomings. The structure and composition of human teeth are similar to bone, which are easily to obtain and have slight rejection and good biocompatibility. At present, it is reported that the autogenous teeth could be used for bone substitutes. This article reviews the application of autogenous teeth bone in dental implantation. References | Related Articles | Metrics

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Research Progress in Prediction of Dental Caries Based on FQ-PCR SUN Tong-zheng, YANG Fang 2019, 35(1): 13-15.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.003 Abstract ( 250 )   PDF (776KB) ( 286 )   Dental caries is a chronic infectious disease that occurs in dental tissues. It develops in a progressive irreversible manner and eventually leads to the defect or loss of dental tissues. Caries is extremely high incidence in the population, affecting the patient's life quality seriously. It is critical to study the etiology of caries and prevention. FQ-PCR can quantitatively study the etiology and pathogenesis of dental caries and find out the micro-flora closely related to dental caries and provide a new method for the prevention and treatment of dental caries. References | Related Articles | Metrics

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Research Progress on Three-dimensional Occlusion Model SU Li, HUANG Xiao-feng 2019, 35(1): 16-19.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.004 Abstract ( 382 )   PDF (779KB) ( 564 )   The application of three-dimensional digital technology in stomatology is becoming more and more extensive. Digital modeling is obviously the important foundation of digital medicine. At present, there are many methods for digital modeling, but different methods have advantages and disadvantages, and there is no standard modeling method. Several kinds of modeling methods that are commonly used in clinic and scientific research are discussed. In this paper, the reliability and accuracy of the methods are reviewed and compared, and the future development of the digital modeling method is prospected. References | Related Articles | Metrics

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Research Progress on Comprehensive Prevention and Treatment of Facial Malformation and Frey Syndrome after Parotid Neoplasms ZHANG Kai, LI Chun-ming 2019, 35(1): 20-22.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.005 Abstract ( 300 )   PDF (772KB) ( 299 )   The facial deformity and the gustatory sweating syndrome (Frey syndrome) are common complications following parotidectomy. The previous studies mostly emphasize on the repair of smaller defects (tumors less than 3 cm) and the prevention of Frey syndrome after parotidectomy, but do not consider the comprehensive prevention. Recently, many scholars conducted a series of studies on the comprehensive prevention and treatment, especially in the large defect (tumors 3-5 cm) after parotidectomy. In order to restore the facial deformity and prevent Frey syndrome, this article makes a summary on this. References | Related Articles | Metrics

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Imbalanced Post-operative Weight Loss in Combined Obesity and Periodontitis Models YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying 2019, 35(1): 23-27.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.006 Abstract ( 187 )   PDF (1312KB) ( 155 )   Objective: To explore the effects of main experimental factors on post-operative weight loss (POWL) when establishing combined obesity and periodontitis models. Methods: Combined models of diet-induced obesity and ligation-induced periodontitis were established. Effects of feeding duration (8, 16 and 30 weeks), diet, ligation and ligation duration (5 and 10 days) on POWL were evaluated by 4-way ANOVA. Results: DIO and periodontitits mice had 70% (10.4% vs. 6.1%, P<0.001) and 30% (9.6% vs. 7.4%, P=0.002) higher POWL, respectively, in contrast to their corresponding controls. As severity of obesity increased, POWL of the DIO mice was elevated and more difficult to recover and POWL difference between DIO mice and the controls was further enhanced. More POWL went with longer ligation duration after specific feeding duration (16 weeks). Conclusion: When establishing combined obesity and periodontitis models, imbalanced POWL occurs within groups of the main experimental factors (including obesity and periodontitis and their severity), which should be critically assessed to minimize its potential disturbance on experimental objectives. References | Related Articles | Metrics

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Effect of Over Expression of NICD Gene on Proliferation of Human Periodontal Ligament Stem Cells LONG Yan, QIU Shen-cai, LI Shu-hui, SHE Yu-xiu, WANG Na, CHEN Xiao-yan, WU Pei-ling 2019, 35(1): 28-32.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.007 Abstract ( 249 )   PDF (1190KB) ( 167 )   Objective: To investigate the effect of Notch1 intracellular domain (NICD) gene on the proliferation of human periodontal ligament stem cells (PDLSCs). Methods: The retroviral particles with over expression of Notch1 (NICD) gene were constructed and transfected into the PDLSCs. The general state of the cells was observed after 48h transfection. PDLSCs/NICD was set as the experimental group, PDLSCs/wt was set as normal group, and PDLSCs/empty vector was set as control group. The expressions of NICD mRNA and protein were detected by Real-time PCR and Western Blot, the proliferation of cells was detected by CCK8 method, and the migration ability of the cells was detected. Results: The expression levels of protein and NICD mRNA in the PDLSCs/NICD group were obviously enhanced in contrast to PDLSCs/wt group and the PDLSCs/empty vector group. Cell migration of PDLSCs/NICD group (40.20±1.738) was significantly higher than that of PDLSCs/vector group (21.20±1.182). Conclusion: Over expression of NICD enhanced the proliferation and migration ability of human PDLSCs to a certain extent. References | Related Articles | Metrics

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Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca2+ Signaling Pathway within Estrogen Microenvironment WU Xiao-ling, ZHENG Qian, LV Jia-ling, ZHAO Xian, XU Jie, YU Jing-hong, XU Xiao-mei 2019, 35(1): 33-37.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.008 Abstract ( 236 )   PDF (1753KB) ( 199 )   Objective: To investigate the effects of estrogen on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) regulated by non-canonical Wnt/Ca2+ signaling pathway. Methods: The third generation of hPDLSCs were divided into blank group, control group, estrogen group, and inhibitor group. ALP activity was measured on the 7th day after osteogenesis induction, the expression of Wnt signaling pathway genes and osteoblast-related genes were detected by qRT-PCR. Results: ALP active in estrogen group and inhibitor group were higher than that in control group (P<0.05). There was no significant difference in ALP expression between estrogen group and inhibitor group (P>0.05). qRT-PCR showed that the expression of Wnt signaling pathway genes, such as β-catenin, CaMKⅡ, and NLK, and osteogenic genes (RUNX2, OCN) in estrogen group and inhibitor group were up-regulated compared with control group (P<0.05). The expression of β -catenin in inhibitor group was lower than that in estrogen group (P<0.05), while the expression of CaMKⅡ and NLK was higher (P<0.05). But osteogenic gene RUNX2 and OCN did not exhibit such difference (P>0.05). Conclusion: Estrogen promotes the osteogenic differentiation of hPDLSCs by enhancing the activation of non-canonical Wnt/Ca2+ signaling pathway. References | Related Articles | Metrics

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Effect of Hypoxia on Proliferation and Differentiation of Human Periodontal Ligament Stem Cells ZHENG Shu-can, ZOU Hui, HUANG Xu-yao, SHAO Hai-bin, ZHOU Yong, ZHANG Rui-fang, LI Chao-hui 2019, 35(1): 38-41.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.009 Abstract ( 283 )   PDF (1642KB) ( 186 )   Objective: To examine the effect of hypoxia on human periodontal ligament stem cells (hPDLSCs). Methods: HPDLSCs were collected and validated. Cells were cultured in normoxia (20% O2) or hypoxia (5%O2) for 5 days. MTT was applied to detect the cell proliferation ability in each day. OCT4, SOX2, and NANOG genes were analyzed by RT-PCR in cells culture in hypoxia at day 1 and day 5. Ki67 staining and crystal violet staining assay were used to detect the self-renewal ability of hPDLSCs. Osteogenic and adipogenic differentiation abilities were investigated after 21-day-induction. Results: The results from MTT assay showed that the proliferation ability of hPDLSCs treated with hypoxia was higher (P<0.05). Hypoxia treatment enhanced the related gene expression at both day 1 and day 5 (P<0.05). SOX2 was enhanced strongly at day 5 (P<0.01). Crystal violet staining assay indicated that colony-forming efficiency was increased in hypoxia group (P<0.05). In addition, hypoxia also enhanced the osteogenic differentiation capacity of hPDLSCs. Osteogenic gene RUNX2 was significantly increased (P<0.05). However, although hypoxia increased the expression of adipogenic gene PPAR γ2 and adipogenic differentiation, the results was not statistically significant. Conclusion: Hypoxic preconditioning of hPDLSCs can increase cell proliferation and expression of stemness-related genes. In addition, hypoxia environment can also improve the osteogenesis differentiation of hPDLSCs, but has little effect on adipogenic differentiation. References | Related Articles | Metrics

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Molecular Mechanisms of β-sitosterol on Oral Squamous Cell Carcinoma Cells SCC9 Apoptosis PEI Hao, LI Hai-peng, HUANG Ying-ying, MA Zhao 2019, 35(1): 42-45.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.010 Abstract ( 292 )   PDF (954KB) ( 130 )   Objective: To investigate the effects and related mechanism of β-sitosterol on oral squamous cell carcinoma cells SSC9. Methods: SCC9 cells were treated by various dose of β-sitosterol, and the cell apoptosis was monitored. The intracellular ROS were analyzed and the mRNA and protein level of NADPH oxidase gp91phox were detected. Knock down of gp91phox in SCC9 was performed to confirm the effects of β-sitosterol on SCC9 cells. Results: Compared with the control group, β-sitosterol induced cell apoptosis in a dose dependent manner (P<0.01). β-sitosterol elevated the intracellular ROS level and mRNA and protein expression of gp91phox in SCC9 cells. Inhibition of gp91phox blocked the effect of β-sitosterol on SCC9 cells. Conclusion: NADPH oxidase gp91phox had an important role in mediating the β-sitosterol-induced apoptotic process in SCC9 cells, which might be a target of OSCC treatment. References | Related Articles | Metrics

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Clinical Research on Forearm Flap and Anterolateral Thigh Flap for Reconstructing Postoperative Defects of Oral Cancer HONG Peng-yu, GAO Lu, QIU Guan-hua, QUAN Hong-zhi, TANG Zhan-gui 2019, 35(1): 46-50.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.011 Abstract ( 256 )   PDF (2724KB) ( 482 )   Objective: To explore the contributions of anterolateral thigh flaps and forearm flaps in the reconstruction of defects caused by oral cancer resection. Methods: Sixty patients who were conducted free flap transfers on account of oral cancer operations, from May 2015 to March 2018, were reviewed by Oral and Maxillofacial Surgery of Xiangya Stomatological Hospital. The reconstruction cases contained 44 anterolateral thigh flaps and 16 forearm flaps. The survival conditions of free flaps, the recovering states of donor sites and the shapes and functions of receiving sites were closely observed and recorded. Results: The overall survival rate of free flaps was 90.0% (54/60), and those of forearm flaps and anterolateral thigh flaps were 87.5% (14/16) and 90.9% (40/44), respectively. The difference between two groups was not statistically significant (P>0.05). After a 6 to 36 months follow-up observation, the restoration effects were mostly satisfied. The difference of survival quality score before and after operation in two groups was statistically significant, respectively (P＜0.05). The difference of survival quality score between two groups was not statistically significant (P>0.05) at least 6 months after surgery. Only 2 patients, who were repaired by forearm flaps, showed distinct scars in donor sites while no complications were observed in the patients repaired by anterolateral thigh flaps. Conclusion: Both anterolateral thigh flap and forearm flap have the superiority of survival rate, shape and functional recovery in lesion. Therefore, they are optimistic choices for repairing defects caused by oral cancer. References | Related Articles | Metrics

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Effects of eIF4E-siRNA on Adhesion and Movement Ability of Tongue Squamous Cell Carcinoma Cells CHEN Chang, PENG Yong-jian, CHEN Yan 2019, 35(1): 51-55.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.012 Abstract ( 245 )   PDF (1430KB) ( 150 )   Objective: To study the effect of eIF4E siRNA on adhesion and movement ability of tongue squamous cell carcinoma cells. Methods: Tongue squamous cell carcinoma cell line SCC25 was infected targeting eIF4E interferred lentivirus. The effect of silence was detected by qRT-PCR and Western blot. Cell proliferation and adhesion were detected by MTT assay. Cell motility was detected by scratch healing test. Cell invasion and migration were measured by Transwell chamber. The expression of N-cadherin, E-cadherin, and matrix degrading enzyme MMP-9 and MMP-2 proteins were detected by Western blot. Results: The levels of eIF4E mRNA and protein expression in SCC25 cells infected with eIF4E interfered with lentivirus were decreased. After silencing eIF4E, the proliferation activity of SCC25 cells was decreased, cell adhesion, cell motility, and the ability to invasion and migration were decreased, the level of E-cadherin protein in the epithelial marker was elevated, the level of N-cadherin protein in the interstitial marker was reduced, and the levels of MMP-9 and MMP-2 protein protein in the cells were decreased. Conclusion: eIF4E siRNA can downregulate the expression of eIF4E in tongue squamous cell carcinoma cells, and inhibit the adhesion, movement, invasion, and migration of tongue squamous cell carcinoma cells. References | Related Articles | Metrics

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A Retrospective Study on Factors Influencing Replantation Effect of Totally Avulsed Young Tooth XIAO Fang, DAI Qun, GAO Shuo-cong 2019, 35(1): 56-59.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.013 Abstract ( 330 )   PDF (773KB) ( 342 )   Objective: To elaborate the correlation between post-traumatic treatment factors and the success of dislocation tooth replantation. Methods: The samples that used to analyze the relation among preservation medium, time, and treatment outcomes were selected from 90 patients in our hospital due to trauma from January 2015 to January 2017. Results: Children from six to twelve were at high risk of tooth dislocation. The most common causes of tooth dislocation included falling on the ground(44.4%)and traffic accident(35.5%). The replantation success rate of immature tooth (80.36%) was higher than that of the mature tooth (59.15%). The success rate (91.49%) of replantation within 30 minutes after injury was higher than those within 30min-2h (68.89%) and 2h (37.14%) after injury. The success rate of tooth stored in milk (93.02%) was higher than that stored in physiological saline (70.00%), kept in patients’ oral (68.18%), and stored in dry medium (34.38%, P<0.05). Conclusion: The best prognosis could be achieved by performing the replantation within 30min after tooth dislocation. The success rate of tooth replantation could be improved by keeping the tooth in milk after the trauma. The success rate of replantation of undeveloped teeth was higher. References | Related Articles | Metrics

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Growth of Mandible after Condylar Reconstruction by Posterior Border of Mandibular Ramus ZHENG De-Xin, LUO Dan, MAN Cheng, JIANG Hai-Tao, CHENG Jiao, GAO Si-Ji 2019, 35(1): 60-63.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.014 Abstract ( 240 )   PDF (1906KB) ( 135 )   Objective: To study the growth of the mandible after condylar reconstruction by the posterior border of the mandibular ramus. Methods: Twenty growing goats were included in this study and divided into group A(n=10)and B (n=10). The left condyles of all animals were surgically removed and reconstructed by the posterior border of the mandibular ramus and fixed to the mandibular ramus by a L-shape miniplate, and the right side were kept intact as controls. Animals in group A were sacrificed at 48 weeks after operation for the measurement of mandibular growth. Five animals in group B were killed at 24 and 48 weeks after operation for histological examination. Results: No difference was found in mandibular length, mandibular height, ramus height, and ramus width between two sides at 48 weeks postoperatively. The reconstructed condylar size increased significantly. A well-organized fibrocartilage was seen at the neocondylar surface and was similar to the normal articular fibrcartilage. Conclusion: The reconstructed condyle by posterior border of mandibular ramus has the potential to grow under functional stimuli. And the results showed that the new condyle seemed to be an alternative and promising method for condylar reconstruction. References | Related Articles | Metrics

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Application of Dexmedetomidine Combined with Sevoflurane Inhalation Anesthesia for Pediatric Dental Treatment LI Shu-rong, ZHAO Er-xian, YUAN Jing-jing 2019, 35(1): 64-66.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.015 Abstract ( 217 )   PDF (754KB) ( 143 )   Objective: To investigate the application of dexmedetomidine combined with sevoflurane inhalation anesthesia in pediatric dental caries. Methods: Sixty children who underwent extensive dental treatment under general anesthesia were randomly divided into control group (group C) and dexmedetomidine group (group D), with 30 cases in each group. All children were induced by sevoflurane. After anesthesia, the dexmedetomidine load was 0.5 μg/kg, and then maintained at 0.5 μg/(kg·h) in group D. However, group C was given an equal dose of normal saline. The children in group C was inhaled sevoflurane to maintain anesthesia and maintained BIS 40-60 during surgery. The incidence of heart rate (HR), mean arterial pressure (MAP), and recovery time of anesthesia and pediatric emergence delirium were recorded. Results: Compared with group C, the pediatric anesthesia emergence delirium (PAED) and modified Children’s Hospital of Eastern Ontario Pain Scale (m-CHEOPS) in group D were significantly lower than those in group C. There were significant differences in MAP and HR between group D at T2, T3 and T4. Conclusion: Dexmedetomidine combined with sevoflurane inhalation anesthesia can reduce pediatric emergence delirium and postoperative pain in children. References | Related Articles | Metrics

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Influence of Digital Impression on Clinical Adaptation of Removable Partial Dentures ZHANG Nan, LIU Qing, LIU Na, CHANG Wen-xiao, KANG Jie, DOU Hai-rui 2019, 35(1): 67-70.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.016 Abstract ( 283 )   PDF (1280KB) ( 349 )   Objective: To investigate the influence of digital impression on the clinical adaptation of removable partial dentures (RPDs). Methods: Forty-seven patients with dentition defect of Kennedy Class Ⅲ or Class Ⅳ were selected, and two RPDs were made for each patient, respectively. The patients were taken impression by silicon rubber (the traditional model group) or intraoral digital scanning (the digital model group). The adaptation of retainers, major connectors, base plates, and the occluding accuracy of RPDs were examined and scored. The scoring results were analyzed by χ2 test. Results: The retainers' adaptation and occluding accuracy of RPDs fabricated by digital models were better than those fabricated by traditional models (P<0.05), and the adaptation of major connectors and base plates fabricated by two methods was not different (P>0.05). Conclusion: The adaptation of RPDs to natural teeth and the occluding accuracy at denture placement could be improved by digital impression combined with 3D printing resin model. References | Related Articles | Metrics

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Establishment of Three-dimensional Finite Element Model of Splinted All-ceramic Crowns for Maxillary Anterior Teeth with Horizontal Alveolar Bone Absorption ZHANG Bin, AN Hong, GUO Chao, YANG Bei-bei, WANG Jia-liang 2019, 35(1): 71-74.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.017 Abstract ( 231 )   PDF (1536KB) ( 174 )   Objective: To analyze the stress distribution of splinted all-ceramic crowns for maxillary anterior teeth with horizontal alveolar bone absorption through three-dimensional finite element model. Methods: Healthy adults were taken as the experimental subjects and CBCT was obtained. Computer-aided software like Geomagic studio, Mimics, SolidWorks, and ANSYS were used to model the three-dimensional finite element by uniform reduction of the alveolar bone and periodontal ligament components. Results: Three-dimensional finite element model of all-ceramic crowns of maxillary anterior teeth with horizontal alveolar bone absorption was established. It included 7 components: all-ceramic splinted crowns, prepared teeth, cortical bone, cancellous bone, mucous membranes, pericementum, and pulp. Conclusion: The model is realistic, and the structural characteristics and mechanical parameters of each component are clear. The loading force can be uniformly transmitted to various parts. It can be used in stress distribution analysis. References | Related Articles | Metrics

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Three Dimensional Finite Element Analysis of Customized Titanium Mesh with Different Thicknesses BAI Li-yun, JI Ping, LI Xian, LI Lin-lin, GAO Hui, WANG Chao 2019, 35(1): 75-79.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.018 Abstract ( 260 )   PDF (2275KB) ( 325 )   Objective: To explore the stress distribution of customized titanium mesh for large bone defect under the loading of 100 N by finite element analysis so as to provide biomechanical guidance for the design of personalized titanium mesh. Methods: The three-dimensional reconstruction of the mandible was reconstructed based on the CBCT scanning data and the individualized titanium mesh with different thicknesses were designed. Then, three-dimensional finite element modeling and finite element mechanical analysis were carried out. Results: Under the loading of 100 N, the stress distribution of titanium mesh was uniform and without stress concentration. With the increase of thickness, the maximum Von Mises stresses of titanium mesh were in a downward trend, the stress of titanium screw was not related to the thickness of titanium mesh. Conclusion: Titanium mesh with 0.3 mm thickness can withstand less power, which may not meet the clinical needs for larger bone tissue defects, especially in posterior teeth. The stress values of the titanium mesh with thickness of 0.4 mm and 0.5 mm are all within the safe equivalent stress range of titanium mesh. They have enough strength and are suitable for clinical needs. Considering that the titanium mesh may result in mucosal irritation that leads to exposure of the membrane, the titanium mesh with a thickness of 0.4 mm is more appropriate for clinical need. References | Related Articles | Metrics

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Experimental Study on Reconstruction of Alveolar Ridge Defects with Personalized Titanium Mesh Based on 3D Printing Technology WANG Chao, XIA Rong, LIU Rui, XIAO Nan-nan 2019, 35(1): 80-83.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.019 Abstract ( 263 )   PDF (1387KB) ( 316 )   Objective: To study the effect of a new type of personalized titanium mesh in the aesthetic reconstruction of alveolar ridge defects in vivo. Methods: Six beagles were selected. According to three-dimensional modeling method, the mandible premolars and part of alveolar bone were virtually resented. Corresponding personalized titanium mesh was rebuilt and designed through software, and the finished products were checked the physical and chemical properties. Bilateral mandibular second and third premolars were extracted respectively. Bilateral mandibular bone defects were designed and reconstructed with bone powder + conventional titanium mesh (CTM group) and bone powder + individualized titanium mesh (PTM group). The operation time, membrane gingival exposure, and postoperative infection were recorded. Four months after surgery, titanium mesh was taken out, and the difference in alveolar ridge height was recorded. Meanwhile, the difference of volume and morphology of alveolar ridge between two methods was compared by micro-CT scanning. Results: The new personalized titanium mesh reduced the duration of GBR surgery by about 30 minutes. There were 2 cases of infection in the CTM group, while only 1 case in the PTM group, which showed a difference of 16.6% between two groups. Four months after surgery, the bone defect in PTM group showed the morphological structure of alveolar ridge. There was a statistically significant difference in alveolar ridge height between two groups (P<0.05). Micro-CT scanning confirmed the results. Conclusion: The new type of personalized titanium mesh has unique alveolar ridge morphology and good physical and chemical properties. References | Related Articles | Metrics

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Expression of JAK2 and STAT3 in Chronic Periapical Periodontitis ZHANG Chen, LIU Jun, ZHAO Han, LIU Ling-shuang 2019, 35(1): 84-88.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.020 Abstract ( 250 )   PDF (2107KB) ( 223 )   Objective: To detect the expression of JAK2/STAT3 signal transduction pathway in chronic apical periodontitis in human and speculate its role in chronic periapical diseases. Methods: Twenty healthy periodontal membranes, 20 periapical cysts, and 20 periapical granuloma specimens were stained by HE. Immunohistochemistry was used to detect the expression of JAK2, p-JAK2, STAT3, and p-STAT3 in different groups. Results: JAK2, p-JAK2, STAT3, and p-STAT3 were slightly expressed in normal tissues, and increased in periapical cysts and granuloma. The differences were statistically significant (P<0.05). The difference between JAK2 and p-JAK2 was statistically significant (P<0.05). There was no significant difference in STAT3 and p-STAT3 between periapical cyst group and periapical granuloma group (P>0.05). Tere was a correlation between JAK2 and STAT3 and p-JAK2 and p-STAT3. Conclusion: The expressions of JAK2, p-JAK2, STAT3, and p-STAT3 were increased in chronic periapical diseases. We speculate that JAK2/STAT3 signaling pathway is involved in the inflammatory process of periapical perioditis and may play an important role in its development. References | Related Articles | Metrics

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Effect of Tapered Glide Path on Shaping Ability of ProTaper Next in Simulated Root Canals HAN Yi, FU Hong-yu, HOU Xiao-mei 2019, 35(1): 89-93.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.021 Abstract ( 271 )   PDF (1036KB) ( 194 )   Objective: To compare the effect of glide path by using ProGlider, HyFlex EDM Glidepath File, RaCe ISO 10, PathFile, and stainless steel K file on the shaping ability of ProTaper Next in simulated root canals. Methods: A total of 40 resin blocks were divided randomly into five groups, each prepared with each glide path file. Furthermore, each canal was prepared by using ProTaper Next till X2. Preparation time for ProTaper Next was recorded. Pre- and postoperative images were superimposed and the material removal from the inner and outer canal walls beginning from 0 mm at the end point of the canal were measured using ImageJ. Centering ability of ProTaper Next was determined, accordingly. Results: Preparation times for ProTaper Next after HyFlex EDM Glidepath File and RaCe ISO 10 were significantly shorter than the others. For centering ability, at the apical portion, the transportation of HyFlex EDM Glidepath File+ProTaper Next was the least, while ststainless steeel K file+ProTaper Next was the most. At the curved portion, HyFlex EDM Glidepath File and RaCe ISO10+ProTaper Next were the least, while the transportation of ProGlider+ProTaper Next increased significantly to overwhelm that of stainless steel K file+ProTaper Next. Conclusion: Glide path exhibits significant impact on shaping ability of ProTaper Next in which the less transportation at the apical portion, the larger taper at the coronal portion, the better shaping ability of ProTaper Next. References | Related Articles | Metrics

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Alternative Splicing of CTLA-4 Exon3 is Regulated by SRSF3 HUANG Jun, GUO Ji-hua, FAN Ming-wen 2019, 35(1): 94-97.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.022 Abstract ( 261 )   PDF (970KB) ( 231 )   Objective: To investigate the effects of SRSF3 on alternative splicing of CTLA-4 exon3. Methods: CTLA-4 minigene was co-transfected with SRSF3 siRNA and SRSF3 plasmid in human embryonic renal epithelial cells (293 cell) respectively to knock down and overexpress the expression of SRSF3. The effects on CTLA-4 splicing isoforms flCTLA-4 and sCTLA-4 were analyzed by RT-PCR. Results: Knock down of SRSF3 increased the expression of flCTLA-4 and inhibited sCTLA-4 product in 293 cells. Overexpression of SRSF3 showed the contrary result. Conclusion: SRSF3 regulates the alternative splicing of CTLA-4 exon 3, down-regulates the expression of flCTLA-4, and increases the expression of sCTLA-4 in human embryonic renal epithelial cells. References | Related Articles | Metrics

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Effects of HU-308 on Biological Properties of MC3T3-E1 Osteoblasts at High Glucocorticoids Concentrations ZHANG Yu, HOU Yu-dong, ZHANG Yun-tao, YANG Zhong-xue, QIAO Lu-hui 2019, 35(1): 98-102.  DOI: 10.13701/j.cnki.kqyxyj.2019.01.023 Abstract ( 212 )   PDF (1715KB) ( 243 )   Objective: To observe the effects of HU-308 on osteoblast treated with high concentration of glucocorticoid. Methods: MC3T3-E1 cells were cultured in the high concentration of glucocorticoid (10-6 mmol/L) and then added the HU-308. Cells in concentration of 10-10, 10-9, 10-8, 10-7, and 10-6 mol/L of HU-308 were set as the experimental groups, and that with only glucocorticoid was set as the control group. The CCK-8 was used to detect the cell proliferation, ALP activity, and mRNA expression of Runx2 and OPG. The morphology of the cytoskeleton was observed by confocal laser scanning microscope when the MC3T3-E1 cells were cultured for 24h. Results: The results of CCK-8 showed that there was statistically significant between the control group and experimental group (P<0.05). The cell proliferation was effectively promoted in the experimental group (10-9, 10-8, 10-7 and 10-6 mol/L) in 4 d (P<0.05). ALP activity detection showed no significant difference between experimental groups and control group (P>0.05). With the concentration of HU-308 increased, cells spreading area increased and the internal frame structure was clearer. The mRNA expressions of Runx2 and OPG were significantly raised at low concentration of HU-308 (P<0.05), however, inhibited at high concentration (P<0.05). Conclusion: HU-308 promoted the proliferation, adhesion, and extension of MC3T3-E1 cells under the action of high concentration of glucocorticoids, and low concentration of HU-308 could promote the expression of Runx2 and OPG mRNA. References | Related Articles | Metrics